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Influence of LY294002 on protein kinase B expression in salivary adenoid cystic carcinoma (SACC) - 83 and SACC-LM cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:1002-0098.2006.06.013
- Key Word:
- 癌,腺样囊性;蛋白激酶类;酶抑制剂
Abstract： 目的探讨特异性抑制剂LY294002对体外培养涎腺腺样囊性癌(SACC)细胞中蛋白激酶B(PKB)表达和转录水平的影响.方法经细胞培养后,应用Wester-blot和RT-PCR实验技术,用酶显法显色、EB染色,将实验数据进行配对t检验.结果SACC-83细胞PKB丝氨酸473(Ser473)的表达和转录水平显著低于SACC-LM细胞PKB(Ser473)的表达和转录水平(P＜0.05);而经LY294002处理的细胞PKB(Ser473)的表达显著低于未经处理细胞PKB(Ser473)的表达(P＜0.01),但两者转录水平差异无统计学意义(P＞0.05).结论体外培养的SACC-83细胞PKB(Ser473)的蛋白表达和转录水平显著低于SACC-LM细胞;LY294002对体外培养细胞中的PKB(Ser473)蛋白表达有明显抑制作用.
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