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The establishment of a carcinogenesis model of oral squamous cell carcinoma in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 1
- DOI:
- 10.3760/j.issn:1002-0098.2006.01.008
- Key Word:
- 癌,鳞状细胞;乳头状瘤病毒,人;烃类,芳香
Abstract： 目的诱导永生化口腔上皮细胞系(HIOEC)细胞恶性转化,建立口腔黏膜上皮细胞体外癌变模型.方法通过化学致癌剂苯并芘[B(a)P]体外诱导HIOEC细胞,逐步筛选到鳞状细胞癌细胞系HIOEC-B(a)P.通过微分干涉显微镜和HE染色观察细胞形态学改变;用软琼脂集落形成及裸小鼠异体皮下成瘤的实验鉴定HIOEC-B(a)P细胞的恶性表型.结果① HIOEC细胞在B(a)P诱导培养后可以在正常生理钙离子浓度、含胎牛血清培养液中生长;②细胞在诱导过程中形态多变,最后稳定为多角形铺路石样上皮细胞;③第93代HIOEC-B(a)P细胞开始具备软琼脂集落形成能力;④ HIOEC-B(a)P第55代细胞在裸小鼠皮下首次形成角化团块,第69代细胞形成分化较好的典型鳞状细胞癌,第74代、第96代细胞均形成与临床病理相似的Ⅰ～Ⅱ级鳞状细胞癌.结论生物因素合并化学致癌因素B(a)P可以诱导永生化口腔黏膜上皮细胞恶性转化,为进一步研究口腔黏膜上皮细胞多因素、多步骤癌变机制提供实验模型.
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