You Position: Home > Paper
The study of hBMP-4 gene modified tissue-engineered bone
( views:164, downloads:20 )
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF STOMATOLOGY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:1002-0098.2003.05.023
- Key Word:
- 克隆,分子;基因治疗;组织工程化骨
Abstract： 目的探讨基因治疗与组织工程方法相结合促进成骨的效果.方法应用亚克隆法构建真核表达载体pEGFP-hBMP-4.贴壁法培养兔骨髓基质干细胞(bone marrow stromal cells,bMSCs),体外转染pEGFP-hBMP-4,pEGFP基因并留置空白对照,以天然型无机骨为支架材料,分别构建组织工程化骨,植入裸鼠皮下(每组6例),4周取材,进行组织学及新骨成骨面积定量分析.结果成功构建了序列完全正确的pEGFP-hBMP-4表达载体.bMSCs可以在天然型无机骨支架网孔内表面贴壁生长.裸鼠体内实验表明术后4周转染pEGFP-hBMP-4基因组支架材料中已大部由新骨充满,新骨面积大于对照组(P<0.05).结论应用hBMP-4基因转染的bMSCs作为种子细胞构建组织工程化骨,可望为骨缺损的修复提供一种更为理想的方法.
- [1]蒋欣泉,张志愿,常庆,等.hBMP-4瞬时转染对兔骨髓基质干细胞生物学行为的影响[J].2003,(1).
- [2]蒋欣泉,陈传俊,张志愿.应用骨髓基质干细胞修复骨缺损的研究进展[J].2002,(1).
- [3]蒋欣泉,张志愿,曹俊,等.绿色荧光蛋白基因转染骨髓基质干细胞的瞬时表达检测[J].2003,(2).
- [4]C H, Evans,P D, Robbins.Genetically augmented tissue engineering of the musculoskeletal system.[J].1999,(367 Suppl).
- [5]J, Bonadio,E, Smiley,P, Patil,etc.Localized, direct plasmid gene delivery in vivo: prolonged therapy results in reproducible tissue regeneration.[J].1999,5(7).
- [6]A S, Breitbart,D A, Grande,J M, Mason,etc.Gene-enhanced tissue engineering: applications for bone healing using cultured periosteal cells transduced retrovirally with the BMP-7 gene.[J].1999,42.
- [7]J M, Wozney,V, Rosen,A J, Celeste,etc.Novel regulators of bone formation: molecular clones and activities.[J].1988,242.
- [8]J R, Lieberman,A, Daluiski,S, Stevenson,etc.The effect of regional gene therapy with bone morphogenetic protein-2-producing bone-marrow cells on the repair of segmental femoral defects in rats.[J].1999,81.
- [9]马贵骧,韩巽,王艺明,等.天然型无机骨的制备及其成骨效应的实验研究[J].1996,(1).
- [10]Prockop DJ..Marrow Stromal Cells as Steam Cells for Nonhematopoietic Tissues[J].1997,276(5309).
- [11]S D, Boden.Bioactive factors for bone tissue engineering.[J].1999.