Abstract： Objective:To compare the accuracy of different questionnaires in chronic obstructive pulmonary disease (COPD) screening.Methods:From January to April 2021, 1 153 subjects aged ≥35 years, visiting the Department of Respiratory and Critical Care Medicine, the First Affiliated Hospital of China Medical University, planning to take part in pulmonary function tests, and voluntarily participating in the survey were included. Before the pulmonary function test, the investigators used Lung Function Questionnaire (LFQ), COPD Population Screening Questionnaire (COPD-PS), COPD Diagnostic Questionnaire (CDQ), Revised-CDQ, Primary COPD Diagnosis Acute Exacerbation Risk Assessment Questionnaire (CAPTURE) and COPD Screening Questionnaire (COPD-SQ) to screen COPD patients. Participants were divided into the COPD group and the non-COPD group based on the global initiative for chronic obstructive lung disease (GOLD) 2021 guideline. The receiver operating characteristic (ROC) curve was used to evaluate the screening accuracy of the questionnaires.Results:A total of 720 patients were effectively enrolled, of which 150 were diagnosed with COPD, with a detection rate of 20.83%. The sensitivity and specificity of each questionnaire under the previously recommended cutoff values were: LFQ (86.00%, 48.07%), COPD-PS (78.67%, 58.25%), CDQ (73.33%, 57.19%), Revised-CDQ (85.33%, 46.49%), CAPTURE (80.67%, 41.23%) and COPD-SQ (68.00%, 62.98%). The accuracy of COPD-PS was better than COPD-SQ, LFQ was better than CDQ, COPD-SQ, and CAPTURE. The optimal cutoff value for LFQ was ≤16 points.Conclusion:The LFQ and COPD-PS are recommended for their simplicity and effectiveness in COPD screening.