Abstract： Objective To assess the effectiveness of Ho:YAG Laser in the treatment of broncholithiasis.Methods We retrospectively reviewed the clinical data of 6 patients who underwent Ho:YAG Laser lithotripsy in Peking University First Hospital during May 2012 to October 2015.4 females and 2 males, with a median age of 60 years, were enrolled.Among 6 patients, persistent cough ( n =2), hemoptysis(n=2), recurrent pneumonia(n=2) were the main clinical symptoms.Broncholiths were found in the left side in 1 patient and right side in 5 patients.2 broncholiths were located in main bronchus and 4 in segmental bronchus.There were 2 patients with intraluminal broncholiths and 4 with transbranchial broncholiths.All 6 patients received Ho:YAG Laser(0.8-1.2 J pulse energies, 5-10 Hz frequencies, 365 μm laser fibers) under rigid bronchoscopy in general anesthesia and experienced relief of symptoms . Results Complete removal of broncholith was accomplished in intraluminal broncholith group and 1 patient in transbracnhial broncholith group , the other 3 transbracnhial broncholiths were partly removed . Complications included perioperative massive hemolysis ( n =1 ) , bronchoesophageal fistula ( n =1 ) and postoperative pneumonia (n=2), no long term complications were encounted .Conclusion The Ho:YAG were associated with acute complications including fistula , perioperative massive hemolysis , infections and no long-term side effects.It represents a safe and effective therapy option for broncholithiasis .