Analysis on the polymorphisms of human B cell epitopes of 5 specificity antigens in Mycobacterium tuberculosis in China
- Author:
- No author available
- Journal Title:
- Chinese Journal of Tuberculosis and Respiratory Diseases
- Issue:
- 5
- DOI:
- 10.3760/cma.j.issn.1001-0939.2016.05.008
- Key Word:
- 分枝杆菌,结核;表位,B淋巴细胞;多态性,单核苷酸;Mycobacterium tuberculosis;Epitopes,B-lymphocyte;Polymorphism,single nucleotide
Abstract： Objective To study the diversities of human B cell epitopes of five proteins (Mpt83,Mpt70,LpqH,PstS3,GroES) in Mycobacterium tuberculosis isolates from China.Methods We selected 179 strains isolated from patients with tuberculosis in different regions of China,and cultured these strains by L-J medium.The gene sequences of these 5 proteins were amplified and sequenced by PCR.The variations (single nucleotide polymorphisms,SNP) of the DNA sequences were compared and analyzed according to the immune epitope database (IEDB).The synonymous mutation rate (dS),non-synonymous mutation rate (dN) and dN/dS values were calculated with MEGA6 software.Results Among the 179 clinical MTBC isolates,7 SNP mutations were found in the mpt83gene.The dN/dS value of the whole mpt83 gene was 0.88.One SNP mutation was found in the mpt70 gene.The dN/dS values of the whole pstS3 gene and non-B epitopes areas were 1.74 and 1.40 respectively.But no mutation was found in the groES gene.Conclusion The mpt83,lpqH and pstS3 genes of 179 MTBC strains contain gene SNPs and human B cell epitopes diversities,while the coding gene and human B cell epitopes of protein mpt70 and groES were conservative.
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