Detection of mutations in the gyrA and gyrB genes associated with fluoroquinolone-resistance among clinical isolates of Mycobacterium abscessus in China
- Author:
- No author available
- Journal Title:
- Chinese Journal of Tuberculosis and Respiratory Diseases
- Issue:
- 7
- DOI:
- 10.3760/cma.j.issn.1001-0939.2015.07.009
- Key Word:
- 分枝杆菌属;喹诺酮类;基因;Mycobacterium;Quinolones;Genes
Abstract： Objective To determine molecular mutations in gyrA and gyrB genes and their association with fluoroquinone-resistance among Mycobacterium abscessus isolates from China.Methods Antimicrobial susceptibility profile of Mycobacterium abscessus isolates was evaluated for resistance to levofloxacin (LFX) and moxifloxacin (MFX).The quinolone resistant determing regions(QRDR) of gyrA and gyrB genes of all the isolates was sequenced and analyzed.Results A total 70 Mycobacterium abscessus isolates were analyzed,including 45 (64％) M.abscessus subsp.abscessus and 25 (36％) M.abscessus subsp.massiliense.Analysis of antimicrobial susceptibility profile showed 97％ (n =68) of isolates were resistance to LFX and 14.3％ (n =10) of isolates were resistance to MFX.All the MFX-resistant strains were resistant to LFX.Cross-resistance to LFX and MFX was noted in 14.3％ (n =10) of isolates.Sequencing analysis revealed that the Ser83Ala substitution in the gyrA gene and Lys447Arg and Ser464Asn substitutions in the gyrB gene were found in all the isolates from both M.abscessus subsp.abscessus and M.abscessus subsp.massiliense.No mutation was found to be associated with cross-resistance of M.abscessus to LFX and MFX in the entire gyrA and gyrB gene.Conclusions Our data suggest that there is no clear correlation between the type of mutation in the gene gyrA and gyrB,and the MIC levels of LFX and MFX for resistant M.abscessus strains.
- [1]Alvaro, Hernández,María B, Sánchez,José L, Martínez.Quinolone resistance: much more than predicted.[J].2011,2.
- [2]J S, Wolfson,D C, Hooper.The fluoroquinolones: structures, mechanisms of action and resistance, and spectra of activity in vitro.[J].1985,28(4).
- [3]Fernanda Monego,Rafael Silva Duarte,Sueli Massumi Nakatani,etc.Molecular identification and typing of Mycobacterium massiliense isolated from postsurgical infections in Brazil[J].2011,15(5).
- [4]Nie, W.,Duan, H.,Huang, H.,etc.Species identification of Mycobacterium abscessus subsp. abscessus and Mycobacterium abscessus subsp. bolletii using rpoB and hsp65, and susceptibility testing to eight antibiotics[J].2014,25(1).
- [5]V, Revel,E, Cambau,V, Jarlier,etc.Characterization of mutations in Mycobacterium smegmatis involved in resistance to fluoroquinolones.[J].1994,38(9).
- [6]Vinicius Calado Nogueira, de Moura,Marlei Gomes, da Silva,Karen Machado, Gomes,etc.Phenotypic and molecular characterization of quinolone resistance in Mycobacterium abscessus subsp. bolletii recovered from postsurgical infections.[J].2012,61.
- [7]Seidu, Malik,Melisa, Willby,David, Sikes,etc.New insights into fluoroquinolone resistance in Mycobacterium tuberculosis: functional genetic analysis of gyrA and gyrB mutations.[J].2012,7(6).
- [8]Martin A,Jureen P,Von Groll A.Fluoroquinolone resistance in Mycobacterium tuberculosis and mutations in gyrA and gyrB.[J].2009,53(10).
- [9]Guillemin I,Jarlier V,Cambau E.Correlation between quinolone susceptibility patterns and sequences in the A and B subunits of DNA gyrase in mycobacteria.[J].1998,42(8).
- [10]Choi,G.-E.,Shin,S.J.,Won,C.-J.,etc.Macrolide treatment for mycobacterium abscessus and mycobacterium massiliense infection and inducible resistance[J].2012,186(9).
- [11]Paramasivan CN,Rahman F,Sulochana S.In vitro activity of fluoroquinolones against Mycobacterium tuberculosis.[J].2005,17(2).
- [12]Bryant,J.M.,Grogono,D.M.,Greaves,D.,etc.Whole-genome sequencing to identify transmission of Mycobacterium abscessus between patients with cystic fibrosis: A retrospective cohort study[J].2013,381(9877).