Changes of Th1/Th2 cytokines in immunocompetent patients with pulmonary cryptococcosis
- Author:
- No author available
- Journal Title:
- Chinese Journal of Tuberculosis and Respiratory Diseases
- Issue:
- 10
- DOI:
- 10.3760/cma.j.issn.1001-0939.2013.10.011
- Key Word:
- 隐球菌病;白细胞介素类;干扰素Ⅱ型;单核细胞;Cryptococcosis;Interleukins;Interferon type Ⅱ;Monocytes
Abstract： Objective To study the changes of Th1/Th2 cytokines in immunocompetent patients with pulmonary cryptococcosis (PC).Methods Twenty immunocompetent patients with PC were identified by histopathological examination and enrolled along with the age-and gender-matched healthy controls.The serum concentrations of interferon-γ (IFN-γ),interleukin-4 (IL-4) and interleukin-12 (IL-12) were measured by enzyme linked immunosorbent assay (ELISA).Peripheral blood mononuclear cells (PBMC) in both groups were isolated and incubated with or without recombinant human IL-12 (rhIL-12) for 48 hours,and the concentrations of IFN-γ and IL-4 in the supernatant were measured by ELISA.Results (1) Serum IFN-γ levels were significantly decreased in the patients compared with the control group[(14.5 ±2.7) vs (81.8 ±9.8) ng/L (t =6.590,P ＜0.01)],while no significant difference was observed in serum IL-12 and IL-4 levels[(2.5 ±0.5) vs (2.52 ±0.6) ng/L and (6.9 ± 1.3) vs (7.3 ± 1.5) ng/L,(t =0.0035 and 0.2136,P＞0.05)].(2) The concentrations of IFN-γand IL-4 in the supernatant of PBMC without rhIL-12 stimulation showed no differences between the 2 groups [(55.7 ± 13.6) vs (51.1 ± 17.5) ng/L and (5.1 ± 0.7) vs (5.0 ± 0.6) ng/L(t =0.2979 and 0.0325,P ＞ 0.05)].(3) Treatment with rhIL-12 stimulated the release of IFN-γ,but the increase in the patients [(4.3 ± 0.5) folds] was less compared with that in the controls [(7.9 ± 1.1) folds] (t =3.01,P ＜0.01),while IL-4 concentration in the supematant of PBMC was not increased in both groups [(0.9 ± 0.4) vs (1.3 ± 0.4) folds (t =0.7240,P ＞ 0.05)].Conclusions Serum Th1 cytokine (IFN-γ) levels may be dampened in immunocompetent patients with PC,without significant change in serum levels of Th2 cytokines (IL-4).Deficiency in the response to IL-12 stimulation of Th1 cells may be one of the underlying mechanisms for the decline in serum IFN-γ levels.
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