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A study on the relationship between single nucleotide polymorphisms of interleukin-22 and susceptibility to pulmonary tuberculosis

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Author:
No author available
Journal Title:
Chinese Journal of Tuberculosis and Respiratory Diseases
Issue:
8
DOI:
10.3760/cma.j.issn.1001-0939.2012.08.010
Key Word:
白细胞介素类;多态性,单核苷酸;结核,肺;疾病遗传易感性;Interleukins;Polymorphism,single nucleotide;Tuberculosis,pulmonary;Genetic predisposition to disease

Abstract: Objective To study the association between single nucleotide polymorphisms (SNPs) of interleukin-22 (IL-22) gene and pulmonary tuberculosis.Methods From January 2009 to December 2010,clinical data of 479 patients with pulmonary tuberculosis in Shenzhen Third People' s Hospital were collected.There were 212 males,and 267 females,aging from 18 to 69 (mean 36 ± 17) years,as well as 358 healthy controls (162 males and 196 females),aging from 18 to 60 (mean 34 ± 13 ) years.The genotype of SNPs (rs1182844,rs2227473,rs2227476,rs2227480,rs2227485,rs2227508) in IL-22 gene were determined with MassARRAY assay,after Hardy-Weinberg equilibrium test,and the allelic frequency and odds ratio were calculated. Peripheral blood mononuclear cells (PBMCs) from patients with different rs2227473 genotypes were stimulated with anti-CD3 and CD28, and then the IL-22 concentration in supernatant was determined with ELSIA.The SNP allelic frequencies between 2 groups were analyzed by chisquare and IL-22 concentration by t-test. Results The frequency of allele G of rs2227473 SNP was significantly higher in tuberculosis group than that in the control group ( x2 = 7.448,P < 0.01,OR =1.509,95%CI =1.121 -2.030). The other 5 SNPs allele frequency were not statistically siguificant between the 2 groups ( x2 =O.528 - 3.571,all P > 0.05 ).The secretion of IL-22 was significantly lower in PBMCs with genotype GG of rs2227473 SNP as compared to that in the others (GA/AA) (t =2.686,P <0.01 ).Conclusions The results suggested that the rs2227473 SNP in IL-22 was associated with the risk of pulmonary tuberculosis.The allele G was the risk factor of pulmonary tuberculosis.The SNP ( rs2227473 )may play an important role in the protective immune process against tuberculosis by affecting the IL-22 expression of PBMCs.

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