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A guinea pig model of latent Mycobacterium tuberculosis H37 Rv infection

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Author:
No author available
Journal Title:
CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
Issue:
9
DOI:
10.3760/cma.j.issn.1001-0939.2010.09.013
Key Word:
分枝杆菌,结核;豚鼠;模型,动物;感染;Mycobacterium tuberculosis;Guinea pig;Models,animal;Infection

Abstract: Objective To establish the guinea pig model of latent Mycobacterium tuberculosis (MTB) H37 Rv infection, and to study the multiplication dynamics of MTB in vivo, and the relationship between latent MTB infection and PPD skin test. Methods Sixty-two guinea pigs were randomly divided into the model group (n =42) and the control group (n =20), and the model group was subdivided into a 4 weeks group (n = 12) , an 8 weeks group ( n =21 ) and a 12 weeks group ( n =9), challenged by 500 CFU H37 Rv with restored toxicity. After 2 weeks challenge, the model groups were treated with isoniazid (INH, 10 mg/kg) + pyrazinamidum aldinamide (PZA, 40 mg/kg) for 4 weeks, 8 weeks and 12 weeks respectively. The natural recurrence of tuberculosis was observed in the model 4 weeks group, and the natural and induced recurrence by dexamethasone was observed in the model 8 weeks group and 12 weeks group. PPD skin test, the pathologic changes, and MTB quantity of organs were observed. Results In the control group, the average MTB quantity of spleen was 3.3 lg CFU after 2 weeks challenge, and the average MTB quantity of spleen and lung in guinea pigs were 4.5 lg CFU and 1.8 lg CFU respectively after 6 weeks challenge, and they reached 5.3 lg CFU and 5.4 lg CFU at 18 weeks respectively. The latent MTB infection of the model 4 weeks group recurred naturally 12 weeks after stopping treatment. The latent MTB infection of the model 8 weeks group recurred naturally and by dexamethasone treatment. The latent MTB infection of the model 12 weeks group did not recur naturally, but dexamethasone induced recurrence. The positive PPD response correlated with recurrence. Conclusions A latent MTB infection model was established successfully by H37 Rv challenge and treatment with INH and PZA. The latent MTB infection may recur naturally or by induction. The PPD response was related to tuberculosis recurrence.

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