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Effects of bone marrow mesenchymal stem cells on bleomycin induced pulmonary fibrosis in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 9
- DOI:
- 10.3760/j.issn:1001-0939.2007.09.011
- Key Word:
- 肺纤维化;博来霉素;干细胞;羟脯氨酸
Abstract： 目的 通过观察骨髓间充质干细胞(MSC)对博来霉素致大鼠肺纤维化动物模型的影响,探讨治疗肺纤维化的新方法.方法 体外分离、培养雄性6周龄SD大鼠的骨髓MSC.将48只雌性SD大鼠随机分为6组,第1～5组经气管注入5.0 mg/kg博来霉素0.3 ml,第1和第3组分别于博来霉素注射第1天和第7天经尾静脉注入雄性大鼠MSC液0.2 ml(细胞数为2.5 ×106个);第2和第4组分别于博来霉素注射第1天和第7天经尾静脉注入等量的磷酸缓冲液0.2 ml;第5组作为模型阳性对照,注射博来霉素后未给予其他处理;第6组作为模型阴性对照,经气管注入等量生理盐水0.3ml代替博来霉素,未给予其他处理.于实验第28天统一处死大鼠,留取肺组织行病理学检查、羟脯氨酸含量测定;提取肺组织的DNA,通过聚合酶链反应(PCR)-琼脂糖电泳法检测雄鼠的性别决定基因(sry基因),以判断外源给予的MSC是否在雌性大鼠肺组织中存在.结果 在博来霉素致肺损伤第1天和第7天给予MSC干预治疗后的大鼠肺脏病理改变均较对照组减轻,肺纤维化程度评分分别为(1.0±0.2)分、(2.5±0.5)分和(1.6±0.5)分、(2.3±0.8)分;肺组织羟脯氨酸含量分别为(83±17)μg/mg、(96±20)μg/mg和(123±32)μg/mg、(127±34)μg/mg,在博来霉素致肺损伤第1天较第7天给予MSC其作用更明显.PCR检测结果显示,在博来霉素致肺损伤第1天给予MSC组的大鼠肺组织可以检测到sry基因.结论 外源给予的MSC可以减轻肺纤维化的形成,在肺损伤早期给予MSC干预的疗效更好.
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