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The protective effects of intermedin 1-53 on oleic acid induced acute lung injury in rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 12
- DOI:
- 10.3760/j:issn:1001-0939.2006.12.005
- Key Word:
- 呼吸窘迫综合征,成人;过氧化,脂质;油酸;中介素1-53
Abstract： 目的 探讨血管活性肽中介素1-53对油酸致急性肺损伤(ALI)的保护作用及其可能机制.方法 将雄性Wistar大鼠24只按随机数字表法分为正常对照组、油酸组和治疗组,每组8只.尾静脉注射油酸(0.2 ml/kg)制备大鼠ALI模型,注射前1 h 及注射后 2 h各腹腔注射中介素1-53(每次2 nmol/kg).6 h后分别行动脉血气分析检查,观察肺组织形态学变化,测定肺湿干质量比值(W/D)、支气管肺泡灌洗液(BALF)中白细胞计数及中性粒细胞分类;测定肺组织髓过氧化物酶活性(MPO)、丙二醛(MDA)和共轭二烯键(C-diene)含量.结果 治疗组动脉血氧分压(PaO2)为[(81.7±4.6) mm Hg,1 mm Hg=0.133 kPa],肺W/D比值为5.49±0.63,BALF中白细胞计数及中性粒细胞分别为(57.9±7.4)×104/ml、0.718±0.085,与油酸组[(60.8±3.2)mm Hg、6.18±0.34、(122.0±16.6)×104/ml、0.878±0.026]比较差异均有统计学意义(q值分别为16.74、3.43、17.23、6.32,P均＜0.05、0.01).结论 中介素1-53能明显降低肺组织的脂质过氧化反应,减轻肺组织的损伤程度,对油酸致大鼠ALI具有一定的保护作用.
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