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DHA-1 type cephalosporinase accompanied with SHV-12 prevailed in clinical isolates of Klebsiella pneumoniae
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 7
- DOI:
- 10.3760/j:issn:1001-0939.2005.07.013
- Key Word:
- 肺炎;克雷伯菌属;β内酰胺酶类;抗药性,微生物
Abstract： 目的研究头孢菌素酶(AmpC)和超广谱β内酰胺酶(ESBLs)在临床分离肺炎克雷伯菌中的流行、表型及其基因特性.方法先后用标准纸片扩散法、三维试验、等电聚焦、酶抑制试验以及微量稀释法等进行表型检测.然后用接合试验、多重聚合酶链反应(PCR)以及基因测序等方法进行分子生物学研究.结果受试的86 株细菌中有4株三维试验阳性,等电聚焦以及酶抑制试验表明这些菌株都产一种等电点(PI)为7.8并具有AmpC性质的β内酰胺酶,基因测序表明和DHA-1型AmpC酶一致;它们同时伴随产生一种PI为8.2的ESBLs,基因测序表明其来源为SHV-12.微量稀释法检测表明上述菌株不但对多种三代头孢菌素耐药,而且出现了对头孢吡肟耐药,但对碳青霉烯类抗生素仍敏感.结论本研究发现同时产DHA-1型高产AmpC酶和SHV-12型ESBLs的肺炎克雷伯菌及其耐药表型.此类菌株的出现,将为临床抗感染治疗带来新的困难.
- [1]佘丹阳,刘又宁.AmpC酶和超广谱β-内酰胺酶在阴沟肠杆菌中的表达及对其耐药性的影响[J].2002,(19).
- [2]宋玮,唐英春,陆坚,等.产质粒介导Ⅰ型头孢菌素酶细菌的耐药性及基因型研究[J].2003,(11).
- [3]Perez Perez FJ,Hanson ND.Detection of plasmid-mediated AmpC beta-lactamase genes in clinical isolates by using multiplex PCR.[J].2002,40(6).
- [4]C C, Sanders,W E, Sanders,E S, Moland.Characterization of beta-lactamases in situ on polyacrylamide gels.[J].1986,30.
- [5]Verdet C,Lagrange PH,Gaillot O,etc.Salmonella enteritidis: AmpC plasmid-mediated inducible beta-lactamase (DHA-1) with an ampR gene from Morganella morganii.[J].1998,42(9).
- [6]Poirel L,Nordmann P,Fortineau N.Plasmid-mediated and inducible cephalosporinase DHA-2 from Klebsiella pneumoniae.[J].2001,47(2).
- [7]K S, Thomson,C C, Sanders.Detection of extended-spectrum beta-lactamases in members of the family Enterobacteriaceae: comparison of the double-disk and three-dimensional tests.[J].1992,36.
- [8]A, Bauernfeind,Y, Chong,S, Schweighart.Extended broad spectrum beta-lactamase in Klebsiella pneumoniae including resistance to cephamycins.[J].1900,17(5).