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Application of multiplex polymerase chain reaction in rapid identification of Mycobacterium bovis BCG
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 11
- DOI:
- 10.3760/j:issn:1001-0939.2003.11.015
- Key Word:
- 牛分支杆菌卡介苗;鉴定;基因缺失区1;聚合酶链反应
Abstract： 目的建立快速鉴定牛分支杆菌卡介苗(BCG)菌株的方法. 方法依据最近研究发现的牛分支杆菌BCG菌株不存在命名为缺失区1(deleted region 1,RD1)的基因区,而其他牛分支杆菌菌株和其他结核分支杆菌复合群(MTC)菌种(结核分支杆菌、非洲分支杆菌和田鼠分支杆菌)存在RD1基因区的遗传学信息,应用多重聚合酶链反应(PCR)检测RD1基因区存在与否, 以鉴别BCG菌株与其他牛分支杆菌菌株及其他MTC菌种. 结果所试5株BCG疫苗生产用标准菌株和近期国内发生的1例小儿BCG接种后全身播散性感染致死病例的2株BCG分离菌株,均缺失RD1基因区;其他牛分支杆菌菌株,包括3株牛分支杆菌标准菌株、5株分别从结核病牛或鹿分离的牛分支杆菌菌株,1株从结核病患者痰标本分离的牛分支杆菌菌株,以及其他MTC菌种,包括结核分支杆菌H37Rv和H37Ra标准菌株、48株从结核病患者痰标本分离的结核分支杆菌菌株、3株非洲分支杆菌标准菌株,均存在RD1区. 结论多重PCR技术用于牛分支杆菌BCG菌株的鉴定简便、快速、特异,适于在临床实验室应用.
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