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Protective effect of Ginkgo biloba extract on acute lung injury induced by lipopolysaccharide in D-galactose aging rats
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 6
- DOI:
- 10.3760/j:issn:1001-0939.2002.06.009
- Key Word:
- 急性肺损伤;脂多糖;银杏叶提取物;衰老
Abstract： 目的观察脂多糖(LPS)诱导D-半乳糖(D-gal)致衰老大鼠急性肺损伤(ALI)及银杏叶提取物(GBE)对其是否有保护作用.方法大鼠24只随机分成两部分,6只为正常对照组;18只经腹腔注D-gal复制衰老动物模型.后者再随机分成三组:衰老对照组(6只);LPS组(6只,静脉注射LPS诱导形成ALI);GBE+LPS组(6只,注LPS前7天开始每天灌胃给GBE一次,按所含黄酮甙计算,8 mg/kg体重,实验当日在给LPS前2 h 再给一次GBE).注LPS后2 h 收集标本待测.结果 D-gal致衰老大鼠较正常大鼠血中超氧化物歧化酶(SOD)及肺组织Na+-K+-ATPase活性均显著降低(P均<0.05),而血中乳酸脱氢酶(LDH)活性升高(P<0.05).衰老大鼠注LPS后2 h 已形成ALI. 肺间质及肺泡中有较多炎性细胞;肺泡灌洗液中蛋白含量及肺通透指数增加;血中乳酸(LD),丙二醛(MDA),一氧化氮( NO),内皮素-1(ET-1),肿瘤坏死因子-α(TNF-α)含量和LDH活性以及肺组织中髓过氧化物酶(MPO)活性,均显著升高;而血中超氧化物歧化酶活性及肺组织Na+-K+-ATPase活性均下降(P<0.05,P<0.01).预先给予GBE可显著地缓解除SOD活性外的上述其它指标的变化(P<0.05).结论 D-gal致衰老大鼠体内抗氧化能力降低.静注LPS可引起衰老大鼠明显的ALI.GBE对LPS诱导衰老大鼠的ALI有显著的保护作用.
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