An experimental study of the effect of bacille calmette-guerin vaccine on the production of a rat asthmatic model and its relation with γδ T cells
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 3
- DOI:
- 10.3760/j:issn:1001-0939.2002.03.015
- Key Word:
- 卡介苗;哮喘;大鼠;γ δ T细胞
Abstract： 目的探讨卡介苗(BCG)对哮喘的预防作用及其与γδT细胞的关系.方法 Wistar大鼠40只,随机分为4组,每组10只,用"冻干皮内注射用卡介苗"给大鼠皮内注射,8周后应用鸡卵清蛋白(OVA)致敏并雾化吸入刺激,制作致敏大鼠模型;测定气道反应性变化,并收集外周血单个核细胞(PBMC)和支气管肺泡灌洗液(BALF);采用流式细胞仪检测γδ T细胞抗原受体(γδ TCR)和αβ TCR阳性T细胞百分率及CD28/γδTCR平均荧光密度比,并用免疫荧光法结合HE染色以及免疫组化法检测γδ T细胞占淋巴细胞的百分率.结果常规哮喘模型组(D组)BALF中可见大量嗜酸细胞[EOS,(2.5±1.1)×108/L],与正常对照组(A组)[(0.0)×108/L]比较,差异有显著性(P<0.01);D组50%时乙酰甲胆碱[PC50,(0.28±0.10)g/L]与A组[(1.36±0.76)g/L]比较,差异有显著性(P<0.01);而BCG免疫后哮喘模型组(C组)BALF中很难见到EOS[(0.0)×108/L],与A组[(0.0)×108/L]比较,差异无显著性(P>0.05),而C组PC50[(1.28±0.77)g/L]与A组[(1.36±0.76) g/L]比较,差异无显著性(P>0.05).BALF中BCG免疫组(B组)γδ T细胞/T细胞为(41.3±6.0)%,γδ T细胞/淋巴细胞为(35.2±3.3)%,而C组γδ T细胞 /T细胞为(47.3±8.5)%,γδT细胞/淋巴细胞为(39.0±6.8)%,D组γδ T细胞/T细胞为(32.4±2.6)%,γδT细胞/淋巴细胞为(28.6±2.4)%,A组γδ T细胞/T 细胞为(27.3±0.8)%,γδT细胞/淋巴细胞为(21.8±1.9)%,四组间比较差异有显著性(P均<0.01);CD28/γδTCR平均荧光密度比B组(0.66±0.08)、C组(0.88±0.26)、D组(0.71±0.15)与A组(0.53±0.06)比较,差异也有显著性(P均<0.01).结论 BCG能预防Wistar大鼠过敏性哮喘模型的形成;γδ T细胞可能存在Th1/Th2模式,并可能是BCG调节免疫和哮喘发病过程中的重要细胞.
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