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Establishment and experimental study on mouse model of Chlamydia pneumoniae pneumonitis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 10
- DOI:
- 10.3760/j:issn:1001-0939.2001.10.005
- Key Word:
- 肺炎衣原体;呼吸道感染;动物模型;病理改变
Abstract： 目的通过复制小鼠肺炎衣原体感染模型,对肺炎衣原体肺炎的发病机制进行初步的探讨.方法以肺炎衣原体鼻内或静脉接种Icr小鼠,通过不同时点(60 d内)处死动物,观察其肺部的病理改变.结果鼻内接种肺炎衣原体后,Icr小鼠可以产生肺部感染,特征性病理改变为斑片状间质性肺炎,早期(7 d内)病变较重,以嗜中性粒细胞浸润为主,并伴有泡沫细胞堆积;后期(14 d以后)病变开始减轻,以嗜中性粒细胞和淋巴细胞混合浸润为主,并逐渐转为以淋巴细胞浸润为主.静脉接种肺炎衣原体后的肺部改变与鼻内接种类似,但病变程度要轻得多,范围也要小得多,恢复正常的时间也较快,两者差异在发病早期更为明显.以聚合酶链反应(PCR)法在接种后的肺组织中间歇性检测到肺炎衣原体DNA.被接种的小鼠可以产生血清IgG抗体.结论给Icr小鼠接种肺炎衣原体可以产生以间质性肺炎为特征的肺部感染,鼻内接种者更为明显.
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