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一氧化氮和白细胞介素1β在急性肺损伤的作用研究
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF TUBERCULOSIS AND RESPIRATORY DISEASES
- Issue:
- 7
- DOI:
- 10.3760/j:issn:1001-0939.2001.07.020
- Key Word:
- No keyword available
Abstract： @@ 采用低血容量性休克后加小剂量内毒素模拟急性肺损伤动物模型,探讨此病理生理过程前炎细胞因子变化趋势.对象与方法 22只新西兰白兔分成4组:(1)低血容量性休克组(6只):急性失血持续1 h,以心排出量低于基线40%为准.(2)内毒素(LPS)组(6只):以低剂量(1 μg/kg)LPS静脉滴注;(3)休克加LPS组(6只):休克恢复90 min后再静注LPS;(4)对照组(4只).实验过程分5个期:基础期; 低血容量休克期:第(1)、(3)组动物从股动脉快速放血,室温下用12 U/ml浓度的肝素保存,维持60 min;休克恢复期:将放出的血在30 min内回输;LPS 2 h期:第(2)、(3)组动物以LPS静注持续30 min;LPS 4 h期.用Respironic公司的P型鼻罩套上的动物口鼻,用美国ML-9841A化学发光法一氧化氮(NO)测量仪监测呼出气中NO浓度的变化.处死动物取右肺泡灌洗液(BALF),用酶联免疫法(ELASA)测定白细胞介素1β(IL-1β)浓度(购自法国Bio-Tech公司);用Griess法测定NO(*)/(2)/NO(*)/(3)浓度.左肺经10%甲醛固定后石蜡切片,HE染色作组织学检查;用免疫组化法检测诱导型一氧化氮合酶(iNOS)的表达活性.应用F检验,结果用眘???
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