Abstract： Objective:To explore the effects of hyperbaric oxygen（HBO）on wound healing in rats with diabetic foot ulcer and its mechanism，so as to provide a point of reference for clinical treatment.Methods:A total of 50 SD rats were randomly divided into HBO group and control group，with 25 rats in each group. Diabetic and diabetic foot ulcer models were made in the two groups. The rats in the HBO group were transferred to a hyperbaric oxygen chamber for treatment，while the rats in the control group were placed in atmospherical pressure air for treatment as a control. Fasting ear venous blood of 2 ml was collected before treatment and in the morning after treatment，and the levels of inflammatory factors interleukin-1（IL-1），IL-6，and tumor necrosis factor-α（TNF-α）in serum were detected by enzyme-linked immunosorbent assay（ELISA）. Meanwhile，the levels of transforming growth factor-β（TGF-β）and vascular endothelial growth factor（VEGF）in peripheral blood of rats were detected by ELISA，and the mRNA expression levels of Notch1 and TGF-β in wound tissue were detected by real-time quantitative PCR.Results:After two courses of treatment，the total effective rate of the HBO group（80.0%）was significantly higher than that of the control group（32.0%），and the difference was statistically significant（ P<0.01）. Compared with those before treatment，the levels of IL-1β，IL-6，and TNF-α in the two groups were significantly decreased after treatment（ P<0.05），and those in the HBO group were significantly lower than the same ones in the control group（ P<0.05）. In addition，compared with those before treatment，the levels of TGF-β and VEGF in the two groups were significantly increased after treatment（ P<0.05），and those in the HBO group were significantly higher than the same indexes in the control group（ P<0.05）. After treatment，the relative expression levels of Notch1 mRNA and protein in wound tissues of diabetic rats in the HBO group were significantly decreased（ P<0.01），and the relative expression levels of TGF-β mRNA and protein were significantly increased（ P<0.01）. Conclusion:HBO treatment can reduce the expression levels of serum inflammatory factors in rats with diabetic foot，promote local angiogenesis，improve the local ischemia，and hypoxia state of ulcers，thus promoting wound healing of diabetic foot ulcer.