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Effects of hyperbaric air saturation exposure on lung oxidative injury and inflammatory cytokines in rats

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Author:
No author available
Journal Title:
Chinese Journal of Health Management
Issue:
6
DOI:
10.3760/cma.j.cn311847-20220516-00159
Key Word:
饱和暴露;减压方案;氧化损伤;炎性细胞因子;Saturation exposure;Decompression scheme;Oxidative injury;Inflammatory cytokines

Abstract´╝Ü Objective:To investigate the effects of hyperbaric air saturation exposure on lung oxidative injury and inflammatory cytokines in rats.Methods:A total of 30 male SD rats were randomly divided into three groups:experimental A group,experimental B group,and control group( n=10 per group). The experimental A group and the experimental B group were pressurized with air to 50 m and exposed to saturation for 24 h,and the decompression time was 59.55 h in total. The control group was placed in a hyperbaric cabin to simulate all experimental processes except being pressurized and decompressed. The behavioral performance of the rats in experimental B group was observed for 2 hours after leaving the cabin. Meanwhile,the behavioral performance of the rats in the experimental A group and the control group was observed for 5 minutes after leaving the cabin,and lung tissue samples were immediately collected to detect the lung wet/dry weight ratio of the rats. The levels of malondialdehyde(MDA)and hydrogen peroxide(H 2O 2),the activities of manganese superoxide dismutase(Mn-SOD)and catalase(CAT),and the levels of interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were measured by the enzyme-linked immunosorbent assay(ELISA)in the experimental A group and the control group. Results:The behavioral performance of rats in the three groups was basically normal,with normal walking and breathing. There was no significant difference in the lung wet/dry weight ratio of the rats between the experimental A group and the control group. The levels of MDA,H 2O 2,IL-1β,and TNF-α in lung tissue of the rats in the experimental A group were significantly increased,while the activities of Mn-SOD and CAT in lung tissue of the rats in the experimental A group were significantly decreased,and the difference was statistically significant compared with the control group( P<0.01). Conclusion:Saturation exposure to hyperbaric air can cause oxidative injury and changes of inflammatory cytokines in lung tissue of rats,which may be related to prolonged exposure to hyperbaric air.

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