Abstract： Objective:To investigate the effect of rotational stimulation on the expression and distribution of α-ENaC in different areas of the inner ears of rats.Methods:With immunohistochemistry staining，the expressions of α-ENaC in different areas of the inner ears of the Sprague-Dawley rats after rotational stimulation were observed.Results:After rotational stimulation，the expressions of α-ENaC in the stria vascularis，the spiral ganglion neurons，peripheral satellite cells，the epithelial and stromal cells of the macula utriculi，the vestibular ganglion neurons of rats were increased，while the distributions of α-ENaC in epithelial and stromal cells of the crista ampullaris were decreased. Moreover，in the spiral ligament，Reissner’s membrane，the epithelial and stromal cells of the macula sacculi，and the epithelial cells of the semicircular canal，the distributions of α-ENaC of the observation group were similar to the control group.Conclusion:The α-ENaC were widely expressed in the inner ears，and the changes of its expression varied among different areas in the inner ears of rats after rotation. Thus，ENaC may be involved in the development of motion sickness through regulating the endolymph balance and vestibular sensation of the inner ears.