Abstract： Objective To investigate the role of miR-200b in the treatment of cerebral hypoxia-ischemia with ganglioside sodium (GM) and hyperbaric oxygen (HBO).Methods Thirty newborn P7 SD rats were randomly selected for the study,6 rats were designated as the control group (or group A) and the remaining 24 received left carotid artery ligation to induce ischemia,and the model of cerebral ischemia/ hypoxia was thus established.Then,the 24 brain injury model rats were divided into 4 groups:the model group (or group B),the HBO combined with GM and TCM rehabilitation group (or group C),the miR-200b inhibitor plus HBO combined with GM and TCM rehabilitation Group (or group D) and the miR-200b agonist plus HBO combined with GM and TCM rehabilitation group (or group E).HE staining,immunohistochemistry and high performance liquid chromatography were used to detect the number of normal nerve cells in the cortex,the expression levels of inflammatory factors and excitatory amino acids.Results The combined treatment with HBO,GM and traditional Chinese medicine rehabilitation therapy could effectively alleviate brain damage induced by hypoxic ischemia,and miR-200b could produce certain synergetic effect,while miR-200b activator could produce certain inhibitive effect(P ＜0.05).The infarct volumes of the offsprings in group C,D and E were significantly lower than that in group B,and that in group D was significantly lower than that in group C and E,and that in group D was significantly lower than that in group C and group E.Statistical significance could be seen,when comparisons were made between them (P ＜ 0.05 or P ＜ 0.01).The number of normal neurons in the cerebral cortex of the offsprings in groups C,D and E was significantly higher than that in group B,and the number of normal neurons in groups C,D and E was significantly higher than that in group B,the number of normal neurons in group D was significantly higher than that in groups C and E,and the number of normal neurons in group C was significantly higher than that in group E.There was also statistical significance,when comparisons were made between them (P ＜0.05 or P ＜ 0.01).The relative expression levels of TNF-a and IL-6 protein in the cerebral cortex of the offsprings in group C,D and E were significantly lower than that in group B,the relative expression levels of TNF-a and IL-6 protein in the cerebral cortex of the offsprings in group D were significantly lower than that in group C and E,and the number of normal nerve cells in group C was significantly lower than that in group E,with statistical significance (P ＜0.05 or P ＜0.01).The levels of Glu and Asp in the cortex of the offsprings in group C,D and E were significantly lower than those in group B,while the levels of Glu and Asp in the cortex of the offsprings in group D were significantly lower than those in group C and E,and the levels of Glu and Asp in group C were significantly lower than those in group E,also with statistical significance (P ＜ 0.05 or P ＜ 0.01).Conclusion HBO + GM + TCM rehabilitation therapy could effectively alleviate cerebral ischemia and hypoxic injury in offspring rats,and the inhibitor of miRNA-200b could produce certain synergistic effect.