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Effect of hyperbaric oxygen on the signal pathway of p38 mitogen-activated protein kinases in rats with diaphragmatic damage induced by sepsis

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Author:
No author available
Journal Title:
Chinese Journal of Nautical Medicine and Hyperbaric Medicine
Issue:
4
DOI:
10.3760/cma.j.issn.1009-6906.2011.04.008
Key Word:
脓毒症;细胞凋亡;高压氧;Sepsis;Apoptosis;Hyperbaric oxygen

Abstract: Objective To explore the effect of hyperbaric oxygen(HBO) on the signal pathway of p38 mitogen-activated protein kinases (MAPK) in rats with diaphragmatic damage induced by sepsis, and also to investigate the protective effect of HBO on diaphragmatic damage. Methods Thirty adult SD rats were randomly divided into 3 groups: Group A (the sham operation group, n = 10), Group B (the sepsis group, n= 10) and Group C ( the HBO group, n = 10). Following anesthesia with choral hydrate, the rats of Group A were cut in the mid line of the abdomen, and were then sutured. Sepsis model was established by using cecal ligation and puncture (CLP) for the animals in group B and C, and the rats in Group C were immediately treated with HBO following surgery. All the rats were sacrificed 16 hours after surgery. Diaphragm muscles on the left side were taken to measure the contracti1e force and diaphragm muscles on the right side were taken to detect the apoptosis rate and the expression of p38 MAPK. Results The apoptosis rate of diaphragm muscles and positive expression rate of p38 MAPK for the rats in Group B increased significantly, when they were compared with those of the rats in Group A. The contractile force of diaphragm muscles decreased with statistical significance, when a comparison was made between the 2 groups (P < 0.01 ). Following HBO therapy, the apoptosis rate of diaphragmatic muscles, the expression rate of p38 MAPK and the contractile force of the animals in group B improved obviously and statistical significance could be doted between the 2 groups (P <0. 0 1 )Conclusions H BO could effectively inhibit the expression rate of p38 M APK, decrease the apoptosis rate of diaphragm muscles, thus alleviating lesion of diaphragmatic muscles induced by sepsis.

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