Abstract： Objective To investigate antifungal activities of 32 extracts from marine microorganisms and changes in fungal spectroscopy in vitro. Methods One hundred and twelve microorganisms were isolated from jellyfish and sea soil of the East China Sea, and 32 compounds were further extracted from the microbial zymotic fluid. In vitro antifungal effects of the 32 compounds were studied with microbroth dilution assay, and changes in fungal spectroscopy were further studied with infra - red and Raman spectroscopy. Results Four compounds, i.e. PHA(Ⅰ) ,PHB(Ⅱ) ,PHC(Ⅲ) ,PHD(Ⅳ), could inhibit the growth of the 7 tested fungi, and had obvious effects on Candida albicans with a MIC value of 16 mg/L. PHA(Ⅰ) was also effective to Candida albicans, and at the same time it could obviously inhibit Candida pseudotropicalis, with the MIC value being 8 mg/L and 32 mg/L Conclusions All the 4 compounds had relatively strong antifungai activity and the spectroscopy of Candida albicans by PHA(Ⅰ) was identical to that of amphotericin B.