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Effects of hyperbaric oxygen exposure on the antigen presentation function of mice peritoneal macrophages in vitro
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF NAUTICAL MEDICINE AND HYPERBARIC MEDICINE
- Issue:
- 4
- DOI:
- 10.3760/cma.j.issn.1009-6906.2005.04.014
- Key Word:
- 高压氧;巨噬细胞;抗原提呈功能
Abstract： 目的探讨高压氧(HBO)暴露对离体小鼠腹腔巨噬细胞(PMφ)抗原提呈功能的影响.方法巨噬细胞分为:常压空气对照组(NA)、HBO组Ⅰ(HBO-Ⅰ)、HBO组Ⅱ(HBO-Ⅱ)以及HBO组Ⅲ(HBO-Ⅲ).各组按相应方案暴露,相应时间点观察巨噬细胞吞噬功能、Fc受体功能、NO分泌量、TNF-α分泌量、淋巴细胞DNA合成状况以及Ia抗原表达的变化.结果经400 kPa 4 h HBO暴露,所测巨噬细胞的各指标均明显下降(P＜0.05);经400 kPa 6 h HBO暴露,所测巨噬细胞的各指标亦明显下降(P＜0.05和P＜0.01),且较400 kPa 4 h HBO暴露变化更为明显.巨噬细胞经200 kPa 4 h HBO处理后,各指标变化不明显.结论一定压力和时程的HBO暴露能显著抑制小鼠腹腔巨噬细胞功能,体现了氧对免疫细胞的直接作用.HBO抑制巨噬细胞抗原提呈功能可能通过抑制其Ia抗原表达来实现.
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