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Experimental study of suppressive effects of intravascular local transfer of I κ Bα gene on neointimal hyperplasia after stent implantation
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF RADIOLOGY
- Issue:
- 8
- DOI:
- 10.3760/j.issn:1005-1201.2002.08.004
- Key Word:
- 支架;转基因;血管内膜;细胞游走抑制;动物,实验
Abstract： 目的观察血管内局部转移腺病毒携带的I κ Bα基因对兔髂动脉内支架植入术后内膜增生的抑制作用.方法 12只杂种新西兰大白兔,24支髂动脉,随机(计算机)分为转基因组、磷酸盐缓冲液(PBS)对照组和空白对照组(各组均为8支髂动脉).转基因组髂动脉于支架植入术后,经多隧道球囊导管输送腺病毒携带的I κ Bα基因行局部转基因治疗;PBS对照组于髂动脉支架植入术后,局部注射PBS;空白对照组则仅行髂动脉内支架植入术.分别于术后1周和4周6只兔重复髂动脉造影,之后再处死动物,取支架植入处动脉作病理检查,分近、中、远3段,测量新生内膜截面积等.结果术后1周和4周造影显示各组的管腔内径间差异均无显著性意义(F=0.05,2.71;P>0.05),但术后4周,各组新生内膜截面积分别为(2.28±0.14) mm2,(3.26±0.25) mm2,(2.80±0.20) mm2,转基因组明显小于对照组(F=5.07,P<0.05).结论血管内局部转移腺病毒携带的I κ Bα基因可以抑制兔髂动脉内支架植入术后的内膜增生,从而可能降低支架植入术后再狭窄的发生率.
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