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Sr2+辅助卵母细胞激活技术改善卵母细胞胞质内单精子注射受精失败患者的妊娠结局
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Obstetrics and Gynecology
- Issue:
- 3
- DOI:
- 10.3760/cma.j.issn.0529-567x.2012.03.012
- Key Word:
- No keyword available
Abstract： 卵母细胞胞质内单精子注射(intra-cytoplasmic sperm injection,ICSI)技术不受精子数量和形态影响,具有受精率高、多精受精率低等优点,已成为辅助生殖领域尤其是治疗男性不育的重要手段.该技术将精子直接注射到卵母细胞胞质内,绕过了自然受精过程中的诸多环节,因此常用于体外受精障碍者.尽管如此,临床上仍有1.29％ ～ 3.00％的ICSI周期存在受精率低甚至受精完全失败[1].研究表明,卵母细胞激活不充分可能是导致ICSI受精失败的重要因素之一[2].辅助卵母细胞激活(assisted oocyte activation,AOA)技术可以有效地克服因卵母细胞激活不足引起的受精失败或受精率低,并能获得正常发育的胚胎[2-3].辅助激活的方法包括电脉冲、机械刺激、乙醇、Ca2+载体、蛋白质合成抑制剂和蛋白磷酸化抑制剂应用等[4-6].近年来有报道,Sr2+已成功应用于诱导小鼠和人类卵母细胞的激活[7-8].本研究拟采用ICSI技术结合Sr2+辅助处理,评估Sr2+作为卵母细胞的激活剂能否有效改善ICSI周期的临床结果,现报道如下.
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