Expression of netrin-1 in placenta from patients with pre-eclampsia and the relation to placental angiogenesis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0529-567X.2006.09.006
- Key Word:
- 先兆子痫;胎盘;新生血管化;病理性,神经生长因子类;肿瘤抑制蛋白质类
Abstract： 目的 探讨轴突导向因子netrin-1在子痫前期孕妇胎盘组织中的表达及其对胎盘新生血管形成中的作用.方法 采用RT-PCR和免疫印迹技术分别检测正常妊娠妇女20例(正常妊娠组)和子痫前期孕妇20例(子痫前期组,其中轻度12例,重度8例)的胎盘组织中轴突导向因子netrin-1 mRNA和蛋白的表达;采用免疫组化并通过抗F8因子抗体检测两组孕妇胎盘组织中的血管密度.结果 (1)正常妊娠组及子痫前期组孕妇胎盘组织中netrin-1 mRNA相对吸光度(A)值分别为0.51±0.08和0.41±0.06,netrin-1蛋白A值分别为26.4±1.8和20.5±1.3.两组分别比较,差异有统计学意义(P＜0.01).子痫前期组轻度和重度孕妇netrin-1 mRNA A值分别为0.48±0.08和0.34±0.07,netrin-1蛋白A值分别为22.8±1.3和18.2±1.0.两者分别比较,差异有统计学意义(P＜0.01).(2)子痫前期组孕妇胎盘血管密度为(54±8)个,正常妊娠组孕妇为(65±10)个,两组比较,差异有统计学意义(P＜0.01);子痫前期组中重度孕妇血管密度为(48±7)个,轻度孕妇为(60±9)个,两者比较,差异有统计学意义(P＜0.01).(3)正常妊娠组孕妇胎盘组织中netrin-1 mRNA及其蛋白表达与血管密度呈正相关关系,相关系数(r)分别为0.67和0.71(P均＜0.01);子痫前期组孕妇胎盘组织中netrin-1 mRNA及其蛋白表达与血管密度呈正相关关系,r分别为0.61和0.70(P均＜0.01);子痫前期组轻度孕妇胎盘组织中netrin-1 mRNA及其蛋白表达与血管密度呈正相关关系,r分别为0.69和0.73(P均＜0.01);重度孕妇胎盘组织中netrin-1 mRNA及其蛋白表达与血管密度呈正相关关系,r分别为0.71和0.75(P均＜0.01).结论 子痫前期孕妇胎盘组织中netrin-1表达下降,可能是胎盘血管密度降低的原因之一.
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