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应用RNA干扰技术研究survivin基因对子宫内膜癌细胞增殖和细胞周期的调节作用
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 7
- DOI:
- 10.3760/j.issn:0529-567X.2006.07.017
- Key Word:
- No keyword available
Abstract： @@ survivin基因是近年来发现的在大多数肿瘤组织中高表达的基因,具有调控细胞周期和细胞凋亡的双重作用,与肿瘤的发生密切相关,抑制其功能将有助于肿瘤的治疗[1].RNA干扰(RNA interference,RNAi)技术是利用双链RNA在转录后水平特异性地抑制基因表达的一项技术,是研究基因功能和基因治疗的有力工具[2].本研究应用RNAi技术,将自行设计和构建包含有针对survivin基因的特异性小分子干扰RNA(siRNA)的重组质粒转染入子宫内膜癌细胞,以观察阻断survivin基因表达对细胞增殖和细胞周期的影响.
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