Expression of heparanase and angiopoietin-2 Mrna in endometriosis
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 5
- DOI:
- 10.3760/j.issn:0529-567X.2006.05.002
- Key Word:
- 子宫内膜异位症;葡糖醛酸糖苷酶;血管生成素2;新生血管化,病理性
Abstract： 目的探讨乙酰肝素酶(Hpa)和促血管生成素2(Ang-2)基因在子宫内膜异位症(内异症)发生、发展中的作用.方法采用逆转录PCR(RT-PCR)技术,检测86例内异症患者(内异症组,其中Ⅰ～Ⅱ期内异症患者25例,Ⅲ～Ⅳ期内异症患者61例)的异位和在位内膜组织及30例非内异症患者(对照组)的子宫内膜组织中Hpa mRNA和Ang-2 mRNA表达.结果(1)内异症组异位和在位内膜组织中,Hpa mRNA阳性表达率分别为62%(53/86)和55%(47/86),两者比较,差异无统计学意义(P＞0.05);对照组内膜组织中Hpa mRNA阳性表达率为27%(8/30),明显低于内异症组,两组比较,差异有统计学意义(P＜0.05).(2)内异症组异位和在位内膜组织中Ang-2 mRNA的阳性表达率分别为71%(61/86)和65%(56/86),两者比较,差异无统计学意义(P＞0.05);对照组内膜组织中Ang-2 mRNA的阳性表达率为33%(10/30),明显低于内异症组,两组比较,差异有统计学意义(P＜0.05).(3)Ⅰ～Ⅱ期内异症患者的异位及在位内膜组织中Hpa mRNA阳性表达率分别为44%(11/25)和32%(8/25),明显低于Ⅲ～Ⅳ期内异症患者异位与在位内膜组织的阳性表达率[69%(42/61)和64%(39/61)],两者比较,差异有统计学意义(P＜0.05).(4)Ⅰ～Ⅱ期内异症患者的异位及在位内膜组织中Ang-2 mRNA阳性表达率分别为60%(15/25)和64%(16/25);Ⅲ～Ⅳ期患者异位与在位内膜组织的阳性表达率分别为75%(46/61)和66%(40/61),不同期别内异症患者异位及在位内膜Ang-2 mRNA阳性表达率比较,差异无统计学意义(P＞0.05).(5)内异症组的异位内膜组织中Hpa mRNA与Ang-2 mRNA的表达呈显著正相关(P＜0.05).结论内异症患者异位和在位内膜组织中Hpa mRNA和Ang-2 mRNA均呈高表达,且二者呈正相关关系;推测Hpa和Ang-2可能与内异症的发生和发展有密切关系.
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