Study of estradiol on treatment of preeclampsia in rat model
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:0529-567X.2005.11.007
- Key Word:
- 先兆子痫;疾病模型,动物;雌二醇;血压;蛋白尿
Abstract： 目的观察雌二醇用于治疗子痫前期大鼠时,对其血压、尿蛋白含量、白细胞黏附分子CD49d的表达、肿瘤坏死因子α(TNF-α)浓度及新生鼠的影响.方法将30只妊娠大鼠随机分为A、B、C 3组,每组10只,于大鼠妊娠第14天,A、B组大鼠尾静脉缓慢滴注内毒素1μg/kg(以2 ml生理盐水溶解),建立子痫前期大鼠模型;C组妊娠大鼠用输液泵经尾静脉输注生理盐水2 ml,作为对照.从模型建立当天起,B组每天给予17β雌二醇(17β-E2)1 mg/kg,肌内注射(17β-E2用无水乙醇溶解,生理盐水稀释)治疗,A、C组每天给予等量生理盐水肌内注射,共6 d.分别于治疗前、治疗后的第1、2、5天测量大鼠血压;治疗前及治疗后第2、5天测定大鼠24 h尿蛋白含量;于妊娠第20天时终止妊娠,同时采血检测白细胞黏附分子CD49d的表达及TNF-α浓度.结果 A组大鼠治疗第5天(妊娠第19天)时,血压为(134.0±2.4)mm Hg(1 mm Hg＝0.133 kPa)、24 h尿蛋白含量(0.79±0.10)mg、黏附分子CD49d表达量及TNF-α浓度均增加;B组大鼠治疗第5天时,血压为(123.3±1.7)mm Hg,24 h 尿蛋白含量为(0.51±0.08)mg,两组血压及24 h尿蛋白含量比较,差异均有统计学意义(P<0.05);A组大鼠白细胞黏附分子CD49d的表达强度较C组明显上升, B组较A组白细胞黏附分子CD49d表达强度明显下降,两两比较,差异均有统计学意义(P<0.05).A组大鼠血浆TNF-α浓度为22.0,C组为11.2,B组为9.3,两两比较,差异也有统计学意义(P<0.05).B组新生鼠体重较A、C组略有下降,但差异无统计学意义(P＞0.05).结论雌二醇可改善子痫前期大鼠的临床症状,对子痫前期大鼠子代生长发育无明显影响.
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