Study on association between gestational diabetes mellitus and sulfonylurea receptor-1 gene polymorphism
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 3
- DOI:
- 10.3760/j.issn:0529-567X.2005.03.005
- Key Word:
- 糖尿病,妊娠;糖尿病,Ⅱ型;受体,药物;钾通道;多态现象(遗传学)
Abstract： 目的探讨与妊娠期糖尿病(GDM)遗传易感性相关的磺脲类药物受体1(SUR1)基因型以及SUR1不同基因型与体重指数(BMI)、胰岛素分泌水平之间的关系.方法采用聚合酶链反应-限制性片段长度多态性(PCR-RFLP)分析方法,检测GDM患者35例(GDM组)、2型糖尿病的女性住院患者35例(T2DM组)、正常健康孕妇35例(正常对照组)的SUR1基因型,包括24内含子(c和t等位基因,cc、ct和tt基因型)与31外显子(A和G等位基因,AA、AG和GG基因型);用放射免疫法和葡萄糖氧化酶法测定GDM组患者空腹血糖、空腹胰岛素(INS0)水平和口服75 g葡萄糖后2 h胰岛素(INS120)水平.结果 (1)GDM组、T2DM组c等位基因频率(分别为70.0%、71.4%)均明显高于正常对照组的(52.9%),2组分别与正常对照组比较,差异均有统计学意义(P<0.05).(2)GDM组、T2DM组A等位基因频率(分别为41.4%、44.3%)均明显高于正常对照组的(24.3%),2组分别与正常对照组比较,差异均有统计学意义(P<0.05).(3)GDM组24内含子cc基因型患者BMI[(29.2±5.6) kg/m2]、INS0[(14.9±8.7) mU/L]、INS120[(40.2±12.1) mU/L]均显著高于ct基因型患者[BMI (25.2±4.6) kg/m2、INS0 (10.1±6.2) mU/L、INS120 (32.7±10.5) mU/L]及tt基因型患者[BMI (24.2±1.7) kg/m2、INS0 (10.0±2.3) mU/L、INS120 (28.8±8.3) mU/L],分别与ct及tt型比较,差异均有统计学意义(P<0.05),稳态模型的胰岛素抵抗指数(3.9±2.5)显著高于ct基因型患者(2.6±1.8),与ct型比较,差异有统计学意义(P<0.05).(4)GDM组31外显子AA基因型患者INS0[(15.4±3.2) mU/L]显著高于AG、GG基因型患者[分别为(12.1±4.5)、(11.5±4.8) mU/L],分别与AG及GG型比较,差异均有统计学意义(P<0.05).结论 SUR1 24内含子等位基因c及31外显子等位基因A可能为GDM及T2DM的易感基因;SUR1 24内含子cc基因型及31外显子AA基因型可能在高胰岛素血症形成中起一定的作用.
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