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Clonality detection of cervical cancer and cervical intraepithelial neoplasia by human androgen receptor gene analysis
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:0529-567X.2004.11.012
- Key Word:
- 克隆,细胞;剂量补偿作用(遗传学);受体,雄激素;宫颈肿瘤;宫颈上皮内瘤样病变
Abstract： 目的通过分析人雄激素受体(human androgen receptor, HUMARA)基因,检测宫颈癌及宫颈上皮内瘤变(CIN)组织的X染色体失活方式,从而判断其克隆状态.方法对26例宫颈浸润性癌(宫颈癌组)、31例CIN(CIN 组)及33例正常宫颈(正常宫颈组)组织标本,提取其DNA,经甲基化敏感性限制性核酸内切酶Hha Ⅰ消化后,对HUMARA基因片段行PCR扩增并电泳,通过观察电泳条带判断其克隆状态.结果 HUMARA基因的杂合率为89%. 宫颈癌组、CIN组及正常宫颈组组织的单克隆率分别为92%、38%和10%,宫颈癌组分别与CIN组及正常宫颈组比较,差异均有极显著性(P<0.001),CIN组与正常宫颈组比较,差异有显著性(P=0.012).宫颈癌组中,临床分期Ⅰ、Ⅱ、Ⅲ期组织的单克隆率分别为6/7、90%(9/10)及7/7,两两比较,差异均无显著性(P>0.05).CIN组中,CIN Ⅰ、Ⅱ、Ⅲ组织的单克隆率分别为20%(2/10)、38%(3/8)及63%(5/8),呈逐级增高趋势,但各级CIN间比较,差异均无显著性(P>0.05).结论宫颈癌是癌细胞克隆性增殖的结果.对单克隆状态的CIN应给予积极干预或严密随访.HUMARA基因分析可能成为研究宫颈癌发生机制的一种分子生物学检测手段.
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