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Correlation between local hormones and CD36 transcription level in women with polycystic ovary
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 9
- DOI:
- 10.3760/j.issn:0529-567X.2004.09.005
- Key Word:
- 多囊卵巢综合征;抗原,CD 36;睾酮;胰岛素
Abstract： 目的探讨CD 36 在多囊卵巢(PCO)发病中的局部分子机理.方法选择因不同指征行腹腔镜手术的典型多囊卵综合征(PCOS)患者8例(PCOS组,双侧卵巢标本共16份),不对称性PCO患者12例(PCO组,PCO标本12份,正常卵巢标本8份,其余卵巢未分离出卵泡),非内分泌性原发或继发不孕患者15例(对照组,卵巢标本19份,其余卵巢未分离出卵泡).提取卵泡液,测定卵泡液中睾酮、硫酸脱氢表雄酮(DHEAS)、雄烯二酮(A 2)和胰岛素水平;采用RT-PCR半定量方法,检测卵泡内膜细胞CD 36 mRNA的表达水平.结果 PCOS组患者卵巢组织中CD 36 mRNA的相对表达量为0.21±0.05,PCO组患者PCO组织中CD 36 mRNA的相对表达量为0.24±0.07,均明显低于PCO组患者正常侧(0.83±0.08)和对照组卵巢组织(0.83±0.13),差异有极显著性( P <0.01);PCO卵泡液中睾酮、胰岛素水平较正常卵巢组织明显升高,差异有极显著性( P <0.01);卵巢局部睾酮、胰岛素水平与卵泡内膜细胞CD 36 mRNA的表达水平呈负相关( P <0.01).结论 CD 36 在转录水平的下降,可能通过卵巢局部激素(睾酮、胰岛素)水平的变化,参与PCO的发生.
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