Expression of pituitary tumor-transforming gene in endometrial carcinoma
- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 8
- DOI:
- 10.3760/j.issn:0529-567X.2004.08.010
- Key Word:
- 肿瘤蛋白质类;子宫内膜肿癌;成纤维细胞生长因子2;新生血管化,病理学;免疫组织化学;聚合酶链反应
Abstract： 目的检测子宫内膜癌组织中垂体肿瘤转化基因PTTG的表达,并分析其与碱性成纤维细胞生长因子(bFGF)蛋白表达和微血管密度(MVD)计数的相关性,探讨其在子宫内膜癌发生、发展中的作用.方法采用RT-PCR技术,检测50例子宫内膜癌组织中PTTG mRNA的表达,并进行半定量分析;应用免疫组化链霉菌抗生物素蛋白-过氧化酶连接(SP)法,检测PTTG和bFGF蛋白的表达,CD34标记血管内皮细胞并计数肿瘤间质MVD;结合临床病理特征、bFGF蛋白及MVD计数进行分析.另选择15例增生性子宫内膜和12例正常子宫内膜组织作为对照.结果子宫内膜癌组织中PTTG基因呈过度表达,内膜癌组织中PTTG mRNA的阳性表达率及平均表达水平(分别为96%,0.84±0.08)显著高于增生性子宫内膜(分别为60%,0.78±0.06)及正常子宫内膜组织(分别为33%,0.48±0.12),3组间分别比较,差异均有极显著性(P＜0.01);内膜癌组织中PTTG蛋白的阳性表达率(70%)明显高于增生性子宫内膜(40%)及正常子宫内膜组织(17%),3组间分别比较,差异也均有极显著性(P＜0.01);PTTG基因的表达与手术病理分期、淋巴结转移及肌层浸润有明显相关性(P＜0.05),且其在子宫内膜样腺癌组织中的表达显著高于其他类型的内膜癌组织(P＜0.05);PTTG基因的表达与年龄及病理分级无关(P＞0.05).子宫内膜癌组织中,bFGF蛋白表达阳性、阴性标本中,PTTG mRNA表达水平分别为0.86±0.07和0.80±0.06,PTTG蛋白表达阳性率分别为87%和42%,分别比较,差异均有极显著性(P＜0.01);PTTG蛋白表达阳性、阴性标本中,MVD计数分别为(62±18)个和(51±12)个.子宫内膜癌组织中PTTG基因的表达与bFGF蛋白表达及MVD计数呈正相关(P＜0.05).结论子宫内膜癌组织中PTTG基因过度表达参与了子宫内膜癌的发生及演进过程.PTTG基因可能通过诱导bFGF蛋白表达,促进肿瘤的形成及内膜癌组织中新生血管的生成.
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