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cDNA microarray analysis of gene expression in acquired methotrexate-resistant of human choriocarcinoma
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 6
- DOI:
- 10.3760/j.issn:0529-567X.2004.06.009
- Key Word:
- 绒毛膜癌;甲氨蝶呤;抗药性,肿瘤;肿瘤细胞,培养的;基因 ,MDR;寡核苷酸序列分析
Abstract： 目的探讨人绒毛膜癌获得性甲氨蝶呤(MTX)耐药相关基因.方法首先采用剂量递增间歇诱导的方法,对人绒毛膜癌细胞株JAR细胞用MTX长期诱导培养,使其获得稳定的MTX耐药表型(命名为JAR/MTX细胞),然后用包含1000个来源于中国汉族人cDNA克隆的基因表达谱芯片,对JAR/MTX细胞与其母系JAR细胞进行基因表达谱的比较.结果历时1年耐药诱导后,JAR/MTX细胞获得对MTX稳定的耐药性,耐药指数7.3.两张基因芯片结果一致的差异表达基因共9条,其中比例大于2的明显上调基因有:红细胞生成素相关细胞特异亚单位1、硫氧还蛋白还原酶1、平滑肌分化特异性蛋白和真核细胞翻译延长因子2;比例小于0.5的明显下调基因有:胰岛素受体、可溶性载体家族1成员3、亚精胺或精胺N1-乙酰氨基转移酶、β-血红素和假设蛋白.结论 JAR/MTX细胞经MTX长期诱导后出现耐药表型,涉及了多个基因表达的改变.
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