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Expression of vascular endothelial growth factor and its receptors, flt-1 and kinase insert domain-containing receptor in normal human endometrium during menstrual cycle
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0529-567X.2002.12.009
- Key Word:
- 内皮生长因子;淋巴因子;受体,生长因子;受体蛋白质酪氨酸激酶类;月经周期;子宫内膜
Abstract： 目的检测血管内皮生长因子(VEGF)及其受体fms样酪氨酸激酶(flt-1)、含插入区的激酶受体(KDR)在正常子宫内膜中的表达,探讨其在子宫内膜血管生成及内膜生长分化中的作用.方法采用免疫组织化学及原位杂交方法,对50例妇女正常月经周期子宫内膜中VEGF、flt-1、KDR蛋白质及mRNA进行检测,用蛋白印迹法分析子宫内膜中VEGF亚型,用内皮细胞标志物Ⅷ因子标记内膜微血管密度.结果 VEGF、flt-1、KDR蛋白质及mRNA主要分布在正常子宫内膜血管内皮细胞及腺上皮细胞,VEGF、flt-1在分泌中期、月经期的表达较强, KDR在增生中期即达高峰,三者在增生早期的表达均最低.分泌期血管壁面积及血管腔面积大于增殖期 (P<0.05),血管密度无明显周期性改变(P>0.05).正常子宫内膜组织4种VEGF蛋白亚型中,以VEGF121、VEGF165为主,其免疫条带积分吸光度明显高于VEGF189 、VEGF206 (P<0.05).结论 VEGF、flt-1、KDR蛋白质及mRNA在正常子宫内膜中的表达呈明显周期依赖性,分泌期表达增强提示其可能参与胚胎着床,月经期表达增加可能与此期子宫内膜脱落缺氧有关.
- [1]E.埃尔南德斯 (美)B.F.阿特金森 袁耀萼.临床妇科病理学[M].1998.
- [2]Shifren J L,Tseng J F,Zaloudek C J,etc.Ovarian steroid regulation of vascular endothelial growth factor in the human endometrium: implications for angiogenesis during the menstrual cycle and in the pathogenesis of endometriosis.[J].1996.
- [3]Abu Jawdeh-G,Iruela Arispe-ML,Rodriguez Manzaneque-JC.Endometrial endothelial cells express estrogen and progesterone receptors and exhibit a tissue specific response to angiogenic growth factors.[J].1999,6(2).
- [4]Hanahan D..CELL BIOLOGY: Signaling Vascular Morphogenesis and Maintenance[J].1997.
- [5]Ferrara N,Chen H,Keyt BA,etc.Identification of vascular endothelial growth factor determinants for binding KDR and FLT-1 receptors. Generation of receptor-selective VEGF variants by site-directed mutagenesis.[J].1996,271(10).
- [6]Plouet J.,Moro F.,Mazarguil H.,etc.Extracellular Cleavage of the Vascular Endothelial Growth Factor 189 Amino Acid Form by Urokinase is Required for Its Mitogenic Effect[J].1997,272(20).
- [7]Giudice LC,Irwin JC,Giaccia AJ,etc.Hypoxia and cAMP stimulate vascular endothelial growth factor (VEGF) in human endometrial stromal cells: potential relevance to menstruation and endometrial regeneration.[J].1999,84(6).
- [8]Risau W,Marti HH.Systemic hypoxia changes the organ-specific distribution of vascular endothelial growth factor and its receptors.[J].1998,95(26).