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Experimental study of anti-vascular endothelial growth factor hairpin ribozyme gene inhibiting growth of xenografted tumor of ovarian cancer cells
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 11
- DOI:
- 10.3760/j.issn:0529-567X.2002.11.014
- Key Word:
- 内皮生长因子;淋巴因子;RNA,催化;卵巢肿瘤;肿瘤,实验性
Abstract： 目的采用抗血管内皮生长因子(VEGF)发夹状核酶基因,阻断卵巢癌中VEGF的自分泌和(或)旁分泌通路,以检测抗VEGF发夹状核酶基因对卵巢癌细胞VEGF表达及其肿瘤生长的影响.方法采用脂质体介导的方法,将自行设计和构建的抗VEGF发夹状核酶基因真核表达载体转染人卵巢癌细胞SKOV3,采用氨基糖甙庆大霉素(G418)筛选获得阳性克隆;RNA斑点杂交检测核酶基因在卵巢癌细胞中的表达;逆转录-聚合酶链反应(RT-PCR)技术检测转染前后卵巢癌细胞中VEGF的表达;透射电子显微镜观察卵巢癌细胞超微结构改变;观察裸鼠皮下成瘤实验检测转染前后细胞的致瘤能力的变化.结果转染核酶基因后的卵巢癌细胞VEGF mRNA表达明显降低;透射电子显微镜观察出现凋亡细胞;裸鼠致瘤能力减弱,肿瘤组织中VEGF表达及血管形成减少.结论抗VEGF发夹状核酶基因可显著抑制卵巢癌细胞VEGF表达,通过减少血管形成抑制肿瘤生长,为进一步开展肿瘤血管靶向基因治疗,提供了实验依据.
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