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Study on the correlation of the cytokine secreted by the endometrium focus and the microenvirment of the fleument fluid
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- Author:
- No author available
- Journal Title:
- CHINESE JOURNAL OF OBSTETRICS AND GYNECOLOGY
- Issue:
- 12
- DOI:
- 10.3760/j.issn:0529-567X.2001.12.006
- Key Word:
- 子宫内膜异位症;细胞活素类;微循环
Abstract： 目的探讨子宫内膜异位症(内异症)病灶分泌的细胞因子与腹腔液之间的关系及其对生殖的影响.方法对因内异症进行手术治疗或不孕症行腹腔镜检查的妇女43例,其中内异症24例(内异症组)及正常盆腔19例(对照组),采用单核细胞趋化实验分析、斑点杂交分析、酶链免疫吸附测定及胞浆游离钙浓度([Ca2+]i)测定等方法,测定两组患者的腹腔液、腹腔巨噬细胞及异位内膜细胞的特性,并比较两组腹腔液及异位内膜细胞对精子活动力及鼠胚胎卵裂率的影响.结果内异症组及对照组患者腹腔液趋化单核细胞移动的距离分别为(87.2±9.4) μm、(51.9±3.7) μm,两组比较,差异有极显著性(P<0.01);腹腔巨噬细胞数分别为(1.47±0.87)×106/ml、(0.67±0.73)×106/ml(P<0.01);异位内膜细胞可表达低水平的单核细胞趋化蛋白(MCP)-1[吸光度(A)值为18.34±3.95],经白细胞介素(IL)-1β及肿瘤坏死因子(TNF)-α刺激后,其分泌MCP-1mRNA A值分别为56.84±3.21、41.58±2.48,刺激前后比较,差异有极显著性(P<0.01);内异症组腹腔液及异位内膜细胞明显抑制精子的活动力和鼠胚的发育.结论异位症病灶与腹腔液微环境相互影响,腹腔巨噬细胞和异位症病灶合成及分泌某些细胞因子的能力增强,同时可干扰生殖过程的某些环节.
- [1]高颖,罗丽兰,何福仙.异位子宫内膜细胞的凋亡与增殖的研究[J].1999,(9).
- [2]M B, Furie,G J, Randolph.Chemokines and tissue injury.[J].1995,146.
- [3]Stacie J. Weil,Shiyi Wang,Maria Claudia Perez,etc.Chemotaxis of macrophages by a peritoneal fluid protein in women with endometriosis[J].1997,67(5).
- [4]JUERGEN EISERMANN,KAREN B. REGISTER,RONALD C. STRICKLER,etc.The Effect of Tumor Necrosis Factor on Human Sperm Motility In Vitro[J].1989,10(4).
- [5]A, Akoum,A, Lemay,S, McColl,etc.Elevated concentration and biologic activity of monocyte chemotactic protein-1 in the peritoneal fluid of patients with endometriosis.[J].1996,66(1).
- [6]S, Pampfer,B, Moulaert,I, Vanderheyden,etc.Effect of tumour necrosis factor alpha on rat blastocyst growth and glucose metabolism.[J].1994,101.
- [7]Aydin Arici,Engin Oral,Erkut Attar,etc.Monocyte chemotactic protein-1 concentration in peritoneal fluid of women with endometriosis and its modulation of expression in mesothelial cells[J].1997,67(6).
- [8]冯新为.人体中性粒细胞移动的微孔滤膜检测法[J].1985,(3).