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Epigallocatechin-3-gallate Modulates MicroRNA Expression Profiles in Human Nasopharyngeal Carcinoma CNE2 Cells

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Author:: No author available

Journal Title:: Chinese Medical Journal

Issue:: 1

DOI:: 10.4103/0366-6999.196586

Key Word:: Antitumor Agents;Epigallocatechin-3-gallate;MicroRNAs;Nasopharyngeal Carcinoma

Abstract： Background:Epigallocatechin-3-gallate (EGCG) has exhibited antitumor properties in several types of cancers,including nasopharyngeal carcinoma (NPC),but the molecular mechanisms underlying this function remain incompletely understood.The aim of the present study was to characterize the global impact of EGCG on the expression of microRNAs (miRNAs) in NPC cells.Methods:Using microarray analysis,the alterations of miRNA expression profiles were investigated in EGCG-treated CNE2 cells.Furthermore,the target genes and signaling pathways regulated by EGCG-specific miRNAs were identified using target prediction program and gene ontology analysis.Results:A total of 14 miRNAs exhibited ＞2-fold expression changes in a dose-dependent manner after treatment with 20 μmol/L and 40 μmol/L EGCG.Totally 43,49,and 52 target genes from these differentially expressed miRNAs were associated with the apoptosis,cell cycle regulation,and cell proliferation,respectively.A total of 66 signaling pathways,primarily involved in cancer development and lipid and glucose metabolism,were shown to be regulated by EGCG-specific miRNAs.Conclusion:EGCG induces considerable alterations of miRNA expression profiles in CNE2 cells,which provides mechanistic insights into cellular responses and antitumor activity mediated by EGCG.

Reference

[1]Gang LIU,Xinxin Zheng,Yanlu Xu,etc.Long Non-coding RNAs Expression Profile in HepG2 Cells Reveals the Potential Role of Long Non-coding RNAs in the Cholesterol Metabolism[J].2015,(1).
[2]Gang, Lu,Jie, Liao,Guangyu, Yang,etc.Inhibition of adenoma progression to adenocarcinoma in a 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone-induced lung tumorigenesis model in A/J mice by tea polyphenols and caffeine.[J].2006,66.
[3]Li, GY,Li, XL,Wu, MH,etc.Signaling Transduction Network Mediated by Tumor Suppressor/Susceptibility Genes in NPC[J].2009,10(4).
[4]Chih-Yeu, Fang,Chung-Chun, Wu,Hui-Yu, Hsu,etc.EGCG inhibits proliferation, invasiveness and tumor growth by up-regulation of adhesion molecules, suppression of gelatinases activity, and induction of apoptosis in nasopharyngeal carcinoma cells.[J].2015,16(2).
[5]Wang H,Yang CS,Bian S.Green tea polyphenol EGCG suppresses lung cancer cell growth through upregulating miR-210 expression caused by stabilizing HIF-1alpha.[J].2011,32(12).
[6]Y-C, Wang,U, Bachrach.The specific anti-cancer activity of green tea (-)-epigallocatechin-3-gallate (EGCG).[J].2002,22.
[7]Jiang,M.,Zhang,P.,Hu,G.,etc.Relative expressions of miR-205-5p, miR-205-3p, and miR-21 in tissues and serum of non-small cell lung cancer patients[J].2013,383(1).
[8]Tong AW,Nemunaitis J.Modulation of miRNA activity in human cancer: a new paradigm for cancer gene therapy?[J].2008,15(6).
[9]Berking C,Korner H,Tarasov V,etc.Inactivation of miR-34a by aberrant CpG methylation in multiple types of cancer.[J].2008,7(16).
[10]Samuel, Legeay,Marion, Rodier,Laetitia, Fillon,etc.Epigallocatechin Gallate: A Review of Its Beneficial Properties to Prevent Metabolic Syndrome.[J].2015,7(7).
[11]Jian-Min, Yuan.Cancer prevention by green tea: evidence from epidemiologic studies.[J].2013,98.
[12]Iorio MV,Croce CM.MicroRNAs in cancer: small molecules with a huge impact.[J].2009,27(34).
[13]Jiyoung Kim,Xiaowei Zhang,Rieger-Christ, Kimberly M.,etc.Suppression of Wnt Signaling by the Green Tea Compound (-)-Epigallocatechin 3-Gallate (EGCG) in Invasive Breast Cancer Cells.[J].2006,281(16).
[14]Zhang WL,Wu MH,Cao L,etc.Gene expression profiling of nasopharyngeal carcinoma reveals the abnormally regulated Wnt signaling pathway.[J].2007,38(1).
[15]Zhang, Weijun,Yang, Ping,Gao, Fei,etc.Effects of epigallocatechin gallate on the proliferation and apoptosis of the nasopharyngeal carcinoma cell line CNE2[J].2014,8(6).
[16]Fernandez SA,Tsai SY,Stephens JA,etc.Specific tumor suppressor function for E2F2 in Myc-induced T cell lymphomagenesis[J].2007,104(39).
[17]Ellen T, Chang,Hans-Olov, Adami.The enigmatic epidemiology of nasopharyngeal carcinoma.[J].2006,15.
[18]Qin, Qin,Wei, Furong,Li, Baosheng.Multiple functions of hypoxia-regulated miR-210 in cancer.[J].2014,33.
[19]Lu,G.,Picinich,S.C.,Yang,C.S.,etc.Cancer prevention by tea: Animal studies, molecular mechanisms and human relevance[J].2009,9(6).
[20]Chunxia, Chen,Andrew D, Wells.Comparative analysis of E2F family member oncogenic activity.[J].2007,2.
[21]Neoh Hun, Phuah,Noor Hasima, Nagoor.Regulation of microRNAs by natural agents: new strategies in cancer therapies.[J].2014,2014.
[22]Li Y,Guessous F,Zhang Y.MicroRNA-34a inhibits glioblastoma growth by targeting multiple oncogenes.[J].2009,69(19).
[23]Jiang, Yuyang,Yang, Burton B.,Zhang, Yaou,etc.MiR-210 disturbs mitotic progression through regulating a group of mitosis-related genes[J].2013,41(1).
[24]Arola-Arnal, Anna,Bladé, Cinta.Proanthocyanidins Modulate MicroRNA Expression in Human HepG2 Cells.[J].2011,6(10).
[25]Sangtaek Oh,Jungsug Gwak,Seoyoung Park,etc.Green tea polyphenol EGCG suppresses Wnt/β-catenin signaling by promoting GSK-3β- and PP2A-independent β-catenin phosphorylation/degradation[J].2014,40(6).