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Effects and mechanisms of Cryptotanshinone on rats with adjuvant arthritis

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Author:
No author available
Journal Title:
Chinese Medical Journal
Issue:
24
DOI:
10.3760/cma.j.issn.0366-6999.2011.24.030
Key Word:
cryptotanshinone;adjuvant arthritis;lymphocyte proliferation;cytokine

Abstract: Background Cryptotanshinone (CT) is the major active constituent of Salvia miltiorrhiza Bunge.The present study was carried out to investigate the effects of CT on rats with adjuvant arthritis (AA).Methods AA was induced by the metatarsal footpad injection with complete Freund's adjuvant in male Sprague-Dawley rats.The secondary inflammatory reaction was evaluated by hind paw swelling and the polyarthritis index.Activity of interleukin-1 (IL-1) was detected by the concanavalin A-induced thymocytes proliferation assay.The lymphocytes proliferation and IL-2 production were assayed by 3-(4,5-2dimethylthiazal-2yl)2,5-diphenyltetrazoliumbromide (MIT) and activated mouse splenocytes proliferation,respectively.Results Intragastric administration of CT (50 and 100 mg/kg) significantly decreased secondary inflammatory reactions and increased the spleen and thymus index.There was a marked immunologic and inflammatory response in the AA model,which was accompanied by the decrease of thymocyte proliferation and IL-2 production as well as the increase of IL-1 production.CT apparently enhanced thymocyte proliferation and decreased IL-1 production in AA rats.Conclusion These results indicate that CT may exert its anti-inflammatory and immunoregulatory effects through inhibiting lymphocyte proliferation and production of pro-inflammatory mediators.

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