Fibrinogen and P-selectin expression in atherosclerosis model of Sprague Dawley rat
- Author:
- No author available
- Journal Title:
- Chinese Medical Journal
- Issue:
- 22
- DOI:
- 10.3760/cma.j.issn.0366-6999.2011.22.030
- Key Word:
- fibrinogen;P-selectin;atherosclerosis
Abstract： Background Platelet P-selectin plays an important role in inflammation and contributes to thrombosis and hemostasis.Fibrinogen may take part in inflammation,thrombosis,and hemostasis via enhancement of platelet P-selectin expression.This study aimed to discover the correlation between them in atherosclerosis model of Sprague Dawley (SD) rat.Methods Diet-induced atherosclerosis SD rats were adopted as experimental models.The blood from the common abdominal aorta of the rats was obtained to measure the biochemical characteristics and for the check of flow cytometry.Then the aortas were separated carefully,taken out,put into 10％ (w/v) neutral formalin for later use.Then fibrinogen and P-selectin expression were detected by flow cytometry and immunohistochemistry.Results SD rats were induced to atherosclerosis model by high fat diet and vitamin D2 injected.It was discovered that the binding of fibrinogen and the expression of P-selectin on the platelet increase in atherosclerosis model (Group H)than in that in the control group (Group Z),there were closely interrelated.High levels of fibrinogen and P-selectin express on the artery of atherosclerosis rat model.Conclusions Fibrinogen and P-selectin are concerned with atherosclerosis.Fibrinogen can interact with P-selectin in order to contribute to the development of atherosclerosis,high levels of fibrinogen and P-selectin can be regarded as risk factors for markers of atherosclerosis.
- [1]LIN Xue,ZHU Wen-ling,TAN Li,etc.Gender specific association of neonatal characteristics and cardiovascular risk factors on carotid intima-media thickness in a Chinese cohort[J].2010,(17).
- [2]LUO Jing-yun,WU Gui-fu,Xiong Yan,etc.Enhanced external counterpulsation promotes growth cytokines-mediated myocardial angiogenesis in a porcine model of hypercholesterolemia[J].2009,(10).
- [3]TANG Ya-Ling,YANG Yong-zong,WANG Shuang,etc.Mast cell degranulator compound 48-80 promotes atherosclerotic plaque in apolipoprotein E knockout mice with perivascular common carotid collar placement[J].2009,(3).
- [4]JING Li-na,GAO Pei-Yi,LIN Yan,etc.Distribution of wall shear stress in carotid plaques using magnetic resonance imaging and computational fluid dynamics analysis: a preliminary study[J].2011,(10).
- [5]Li YL,Liu ZQ,Qu SL,etc.Comparison of lycopene and fluvastatin effects on atherosclerosis induced by a high-fat diet in rabbits.[J].2008,24(10).
- [6]Thomas W, Jax,Ansgar J, Peters,Gunnar, Plehn,etc.Hemostatic risk factors in patients with coronary artery disease and type 2 diabetes - a two year follow-up of 243 patients.[J].2009,8.
- [7]Sun Z,Hu W,Ni H,etc.Fibrinogen controls human platelet fibronectin internalization and cell-surface retention.[J].2007,5(8).
- [8]Weyrich AS,Schwertz H,Zimmerman GA.Fibrinogen selects selectins.[J].2009,114(2).
- [9]Boehlen F,de Moerloose P,Neerman Arbez M.Fibrinogen and the risk of thrombosis.[J].2010,36(1).
- [10]George James N.,Lyons Roger M.,Morgan Rebecca K..Membrane Changes Associated with Platelet Activation[J].1980.
- [11]Neerman Arbez M,de Moerloose P.Congenital fibrinogen disorders.[J].2009,35(4).
- [12]Li Z,Du X,Delaney MK,etc.Signaling during platelet adhesion and activation.[J].2010,30(12).
- [13]Z M, Dong,A A, Brown,D D, Wagner.Prominent role of P-selectin in the development of advanced atherosclerosis in ApoE-deficient mice.[J].2000,101(19).
- [14]Mathias T, Grebe,Brigitte, Luu,Daniel, Sedding,etc.Fibrinogen promotes early atherosclerotic changes of the carotid artery in young, healthy adults.[J].2010,17.
- [15]Peter C, Burger,Denisa D, Wagner.Platelet P-selectin facilitates atherosclerotic lesion development.[J].2003,101(7).
- [16]Andreas C, Eriksson,Lena, Jonasson,Tomas L, Lindahl,etc.Static platelet adhesion, flow cytometry and serum TXB2 levels for monitoring platelet inhibiting treatment with ASA and clopidogrel in coronary artery disease: a randomised cross-over study.[J].2009,7.
- [17]Yang H,Lang S,Zhai Z.Fibrinogen is required for maintenance of platelet intracellular and cell-surface P-selectin expression.[J].2009,114(2).
- [18]Foiles N,Green D,Schreiner PJ,etc.Elevated fibrinogen levels and subsequent subclinical atherosclerosis: the CARDIA Study.[J].2009,202(2).
- [19]Christopher JL Murray,Alan D Lopez.Global mortality, disability, and the contribution of risk factors: Global Burden of Disease Study[J].1997,349(9063).
- [20]Ersan, Tatli,Fatih, Ozcelik,Meryem, Aktoz.Plasma fibrinogen level may predict critical coronary artery stenosis in young adults with myocardial infarction.[J].2009,16(4).