Identification of proteins that interact with murine cytomegalovirus early protein M112-113 in brain
- Author:
- No author available
- Journal Title:
- Chinese Medical Journal
- Issue:
- 21
- DOI:
- 10.3760/cma.j.issn.0366-6999.2011.21.018
- Key Word:
- cytomegalovirus;brain;M112-113;yeast two-hybrid;protein-protein interaction
Abstract： Background Murine cytomegalovirus (MCMV) early protein M112-113 is involved in viral DNA replication and believed to play a crucial role in the viral pathogenesis.To investigate the biological function of M112-113 protein in the pathogenesis of the brain disorders caused by cytomegalovirus (CMV),a screening for proteins interacting with M112-113 was performed by a yeast two-hybrid system.Methods Bait plasmid pGBKT7-M112-113 was constructed and transformed into AH109 yeast.After confirmation of the expression of MCMV M112-113 in yeast,the bait yeast was mated with a prey yeast containing mouse brain cDNA library plasmid to screen the proteins interacting with M112-113.Interactions between M112-113 and the obtained proteins were verified by yeast two-hybrid assay and chemiluminescent co-immunoprecipitaion.Results Two proteins interacting with M112-113 were identified,including metastasis-associated 1 (MTA1) and zinc finger,CCHC domain containing 18 (ZCCHC18).M112-113 protein could interact with MTA1 or ZCCHC18 in yeast and mammalian cells.Conclusion The interactions of M112-113 with MTA1 or ZCCHC18 may be related to the pathogenesis of MCMV-associated disease in central nervous system.
- [1]Liew CK.,Mackay JP.,Sharpe BK.,etc.Solution structures of two CCHC zinc fingers from the FOG family protein U-Shaped that mediate protein-protein interactions[J].2000,8(11).
- [2]Boppana SB,Fowler KB.Congenital cytomegalovirus (CMV) infection and hearing deficit.[J].2006,35(2).
- [3]C, Sinzger,G, Jahn.Human cytomegalovirus cell tropism and pathogenesis.[J].1996,39.
- [4]Polic B,Jonjic S,Bubic I,etc.Pathogenesis of murine cytomegalovirus infection.[J].2003,5(13).
- [5]Bramanandam, Manavathi,Kamini, Singh,Rakesh, Kumar.MTA family of coregulators in nuclear receptor biology and pathology.[J].2007,5.
- [6]Kylat RI,Ford-Jones EL,Kelly EN.Clinical findings and adverse outcome in neonates with symptomatic congenital cytomegalovirus (SCCMV) infection.[J].2006,165(11).
- [7]U.Setinek,E.Wondrusch,K.Jellinger,etc.Cytomegalovirus infection of the brain in AIDS: a clinicopathological study[J].1995,90(5).
- [8]Kumar R,Bagheri Yarmand R,Wang RA.Emerging roles of MTA family members in human cancers.[J].2003,30(5 Suppl.16).
- [9]Godfrey EW.,Karabekian Z.,Ciocco-Schmitt GM.,etc.Identification and characterization of novel murine cytomegalovirus M112-113 (e1) gene products[J].2002,294(1).
- [10]Y, Shinmura,S, Aiba-Masago,I, Kosugi,etc.Differential expression of the immediate-early and early antigens in neuronal and glial cells of developing mouse brains infected with murine cytomegalovirus.[J].1997,151.
- [11]Li S,Ge H,Boxem M,etc.Increasing specificity in high-throughput yeast two-hybrid experiments.[J].2004,32(4).
- [12]S, Fields,O, Song.A novel genetic system to detect protein-protein interactions.[J].1989,340(6230).
- [13]Tsutsui Y,Kosugi I,Kawasaki H.Neuropathogenesis in cytomegalovirus infection: indication of the mechanisms using mouse models.[J].2005,15(5).
- [14]P, Cinque,R, Marenzi,D, Ceresa.Cytomegalovirus infections of the nervous system.[J].1997,40.
- [15]Y, Toh,S D, Pencil,G L, Nicolson.A novel candidate metastasis-associated gene, mta1, differentially expressed in highly metastatic mammary adenocarcinoma cell lines. cDNA cloning, expression, and protein analyses.[J].1994,269(37).