Prolongation of functional life-span of neutrophils by recombinant verotoxin 2
- Author:
- No author available
- Journal Title:
- CHINESE MEDICAL JOURNAL
- Issue:
- 6
- DOI:
- 10.3760/j.issn:0366-6999.2002.06.022
- Key Word:
- 中性粒细胞;V2毒素;溶血性尿毒症;VTEC相关疾病;neutrophil;verotoxin 2;hemolytic uremic syndrome;verotoxin-producing Escherichia coli-associated disease
Abstract： Objective Verotoxin-producing Escherichia coli (VTEC) strains of serotype O157∶H7 have been implicated in a wide spectrum of diseases, including blood diarrhea, hemorrhagic colitis and hemolytic uremic syndrome (HUS). To further explore the pathological role of verotoxin (VT) in HUS and other VTEC-associated diseases, we investigated the effects of recombinant verotoxin 2 (rVT2) on the biological activity of neutrophils.Methods The technique of flow cytometry, a fluorescent probe 2,7-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF/AM), and the assay of reduced cytochrome c to detect superoxide production were used in this study. Results rVT2 significantly inhibited spontaneous apoptosis in neutrophils. Neutrophils with prolonged survival due to rVT2 maintained various biological functions, such as the expression of adhesion molecules (shading CD62L and raising CD11b/CD18), adherence to human umbilical vein endothelial cells (HUVECs), and generation of superoxide (O2 ). Conclusion Prolongation of the functional life-span of neutrophils by rVT2 may accelerate inflammatory responses at sites of inflammation. This may play a crucial role in neutrophil-mediated tissue injury in HUS and other VTEC-associated diseases.
- [1]Kayoko,Tanji-Matsuba Stephan F.,van Eeden Yuji,etc.Functional Changes in Aging Polymorphonuclear Leukocytes[J].1998,97(1).
- [2]King AJ,Cendoroglo M,Keusch GT,etc.Shiga toxin induces superoxide production in polymorphonuclear cells with subsequent impairment of phagocytosis and responsiveness to phorbol esters.[J].1999,179(2).
- [3]G, Remuzzi,P, Ruggenenti.The hemolytic uremic syndrome.[J].1995,48.
- [4]J, Liu,T, Akahoshi,T, Sasahana,etc.Inhibition of neutrophil apoptosis by verotoxin 2 derived from Escherichia coli O157:H7.[J].1999,67.
- [5]F, Dallegri,L, Ottonello.Tissue injury in neutrophilic inflammation.[J].1997,46(10).
- [6]T M, Carlos,J M, Harlan.Leukocyte-endothelial adhesion molecules.[J].1994,84.
- [7]Bitzan MM,Lin J,Marsden PA,etc.Verotoxin and ricin have novel effects on preproendothelin-1 expression but fail to modify nitric oxide synthase (ecNOS) expression and NO production in vascular endothelium.[J].1998,101(2).
- [8]Kiyokawa N.,Taguchi T.,Mori T.,etc.Induction of Apoptosis in Normal Human Renal Tubular Epithelial Cells by Escherichia coli Shiga Toxins 1 and 2[J].1998.
- [9]M, Morigi,G, Micheletti,M, Figliuzzi,etc.Verotoxin-1 promotes leukocyte adhesion to cultured endothelial cells under physiologic flow conditions.[J].1995,86.
- [10]Etzioni A.Adhesion molecules--their role in health and disease.[J].1996,39(2).
- [11]J M, Bergstein,M, Riley,N U, Bang.Role of plasminogen-activator inhibitor type 1 in the pathogenesis and outcome of the hemolytic uremic syndrome.[J].1992,327.
- [12]P A, van Setten,V W, van Hinsbergh,T J, van der Velden,etc.Effects of TNF alpha on verocytotoxin cytotoxicity in purified human glomerular microvascular endothelial cells.[J].1997,51.