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Researches on Dapagliflozin enhancing podocyte autophagy by activating adenosine monophosphate?activited protein kinase pathway
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- Author:
- No author available
- Journal Title:
- Chinese Journal of Diabetes
- Issue:
- 1
- DOI:
- 10.3969/j.issn.1006-6187.2023.01.012
- Key Word:
- 达格列净;腺苷酸活化蛋白激酶;足细胞;自噬
Abstract： 目的 探讨达格列净通过激活腺苷酸活化蛋白激酶(AMPK)途径增强足细胞自噬.方法 20只4～5周龄SD雄性大鼠随机选取6只为空白对照组(Con),其余有12只成功建模T2DM大鼠,T2DM大鼠随机分为T2DM组及达格列净组(Dap),每组各6只.Dap组予达格列净灌胃治疗4周后,收集标本进行检测,电镜观察肾脏结构变化,免疫荧光、Western blot检测相关蛋白表达水平.结果 电镜下Dap组肾小球区病变轻于T2DM组.Dap组肾重/体重、FPG、FIns、24 h尿总蛋白、24 hUAlb低于T2DM组(P<0.05).与Con组比较,T2DM组自噬标志物微管相关蛋白轻链3(LC3-II/LC3-I)比值、自噬同源蛋白(Beclin-1)、足细胞特异标记蛋白(NPHS2)降低(P<0.05),Dap组p-AMPK/AMPK比值、Beclin-1蛋白、LC3-II/LC3-I比值升高(P<0.05),NPHS2降低(P<0.05).与T2DM组比较,Dap组p-AMPK/AMPK比值、Beclin-1、NPHS2、LC3-II/LC3-I比值升高(P<0.05).结论 达格列净可通过激活AMPK途径增强T2DM大鼠足细胞自噬发挥肾脏保护作用.
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