Abstract: In neuroscience research,neuronal models are crucial tools for elucidating the molecular and cellular processes involved in the disorders of the nervous system.Facilitating easily and reproducibly executable studies,in vitro models,such as the SH-SY5Y cell line culture,help us explore the pathophysiological mechanisms of neurodegenerative diseases;they are also essential for the efficient screening of drugs for treating the diseases of the nervous system(Peng et al.,2021;Strother et al.,2021).