Abstract： Objective:To investigate the therapeutic effect of kaempferol on hypertrophic scar of rabbit ear.Methods:A total of 8 4-months-old New Zealand white rabbits (4 male rabbits, 4 female rabbits) with body weight with body weight of (2.5±0.2) kg, ranging from 2.3 to 2.7 kg were obtained.A total of 8 large eared white rabbits were randomly divided into experimental group and control group by random number table method, with 4 rabbits in each group.After the scar model of rabbit ear was built successfully, the tissue was observed in general, and the expression levels of protein kinase B (Akt) and phosphorylated protein kinase B (p-Akt) in rabbit ear scar tissue were determined by HE staining, Masson staining, immunohistochemical detection and Western-blot detection.Results:After 7 days of administration, scars in the experimental group were lighter in color, flatter in surface, and slightly softer to the touch than scars in the control group.HE staining showed that the epidermis and dermis of the experimental group were slightly thinner than that of the control group, without obvious thickening, inflammatory cells were significantly reduced, collagen fibers were arranged in a regular and orderly manner, and fibroblasts were fusiform, slender and orderly.Masson staining showed excessive accumulation of collagen fibers in the control group, and collagen fiber bundles in the experimental group were thinner and more neatly arranged than in the control group.Immunohistochemical test showed that the expression of p-Akt in rabbit ear scar in the experimental group was significantly lower than that in the control group.Western-blot analysis showed that the expression of p-Akt was significantly lower than that of the control group, but there was no significant difference in the expression of Akt.Conclusions:Kaempferol can inhibit ear scarring by inhibiting the expression level of p-Akt.