Abstract： Objective:To investigate the clinical value of fungal cell free DNA(cfDNA)polymerase chain reaction(PCR)in the diagnosis of invasive fungal infection.Methods:This study was a prospective study, 120 patients with fungal infection, 81 males and 39 females, aged(52.33±2.69)years old, ranging from 35 to 79 years old, admitted to the department of Laboratory Medicine in Ningde Jiaocheng District Hospital from January 2017 to January 2021.All patients were divided into non-invasive fungal infection group( n=39)and invasive fungal infection group( n=81)according to the Diagnostic Criteria for Invasive fungal Infection.The contents of galactomannan(GM)and cfDNA were compared between the two groups, and the diagnostic efficacy of GM and cfDNA was analyzed. Results:The GM content in invasive fungal infection group[(0.74±0.37)OD]was higher than that in non-invasive fungal infection group[(0.21±0.05)OD], and the difference was statistically significant( P<0.001).cfDNA content in invasive fungal infection group[(9 526.33±511.33)GE/ml]was higher than that in non-invasive fungal infection group[(8 014.65±501.45)GE/ml], the difference was statistically significant( P<0.001).The sensitivity of cfDNA(98.77%)was significantly higher than that of GM(86.42%). Conclusions:Fungal cfDNA PCR has high sensitivity in the diagnosis of invasive fungal infection, which is valuable for popularization.