Abstract： Objective:To investigate the effect of clostridium butyricum powder combined with zinc gluconate on children with acute diarrhea, and its effect on serum interleukin-6(IL-6)and interleukin-17(IL-17)levels.Methods:A retrospective study was performed on 78 cases of patients with acute diarrhea who were admitted in the department of GI Medicine, Children′s Hospital of Zhengzhou University from September 2016 to September 2018, including 43 male and 35 female, aged(1.16±0.57)years old, ranging 6 months to 3 years old.Children were randomly divided into the single treatment group and the combined treatment group, with 39 cases in each group.Children in both groups were treated with fluid replenishment, water and electrolyte balance, acid and alkali treatment, and family members were instructed to adjust diet and feeding.The children in the single treatment group were treated with clostridium butyricum powder, and the children in the combination treatment group were treated with zinc gluconate oral liquid on the basis of the single treatment group.The treatment effects and clinical symptom relief of children were counted and compared.Results:The effective rate of the combined treatment group [94.9%(37/39)] was higher than that of the single treatment group[79.5%(31/39)], and the difference was statistically significant( P<0.05); the thickening time[(2.36±0.61)days], anti-diarrhea time[(1.85±0.54)days], pain time[(1.49±0.64)days], and normal defecation time[(3.47±0.51)days] in the combined treatment group were shorter than those in the single treatment group[(4.08±0.74)days, (3.27±0.63)days, (2.33±0.70)days and(5.26±0.82)days], with statistically significant difference( P<0.05); after treatment, the levels of IL-6 and IL-17 in the combined treatment group[(9.66±2.12)pg/ml, (9.78±2.61)pg/ml] were lower than those in the single treatment group[(13.66±2.03)pg/ml, (14.73±2.87)pg/ml], the difference was statistically significant( P<0.05). Conclusion:The combination of clostridium butyricum powder combined with zinc gluconate has an outstanding effect in the treatment of children with acute diarrhea, which can effectively improve the symptoms of children, reduce their inflammatory response, and thereby reduce the pain of children.