Abstract: Objective To investigate the Imipenem resistant Acinetobacter baumannii and the re-lationship between drug resistance and genes of OXA-23. Methods A total of 70 isolates of imipenem re-sistant Acinetobacter bauman(IRAB)and 30 of imipenem sensitive Acinetobacter bauman(ISAB)were col-lected. The sensitivity of antimicrobial agents was detected by broth microdilution method. The polymerase chain reaction(PCR)technique and sequence analysis were used to detect the genes. Results For in addi-tion to add ring element(4. 3% ),low percentages,penicillium acinetobacter baumannii on carbon alkene antimicrobial drug resistant rate are higher,and penicillium carbon alkene antimicrobial sensitive bacteria were more sensitive to most antibiotics,PCR according to the results of this study in IRAB OXA-23 detected 64 strains,detection rate of 91. 4% ,the ISAB OXA-23 check out 8 strains,the detection rate was 26. 7% , and the difference was statistically significant( P<0. 01). Conclusion Carbapene-resistant acinetobacter baumannii has a serious drug resistance,which is closely related to OXA-23.